Organic distributed feedback laser for label-free biosensing of ErbB2 protein biomarker
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Título: | Organic distributed feedback laser for label-free biosensing of ErbB2 protein biomarker |
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Autor/es: | Retolaza, Aritz | Martinez-Perdiguero, Josu | Merino, Santos | Morales-Vidal, Marta | Boj, Pedro G. | Quintana, José A. | Villalvilla, José M. | Díaz-García, María A. |
Grupo/s de investigación o GITE: | Física de la Materia Condensada |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Óptica, Farmacología y Anatomía | Universidad de Alicante. Departamento de Física Aplicada | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Label-free biosensors | Organic lasers | Distributed feedback | Perylenediimides | ErbB2 biomarker |
Área/s de conocimiento: | Óptica | Física Aplicada | Física de la Materia Condensada |
Fecha de publicación: | feb-2016 |
Editor: | Elsevier |
Cita bibliográfica: | Sensors and Actuators B: Chemical. 2016, 223: 261-265. doi:10.1016/j.snb.2015.09.093 |
Resumen: | The human epidermal growth factor receptor 2 (ErbB2) protein plays an important role in human malignancies. Its overexpression has been recognized as a feature of a malignant cancerous phenotype in breast cancer cell lines, and has become one of the most widely investigated clinical indicators of breast, ovarian, gastrointestinal and lung cancers. In this work a vertically emitting organic distributed feedback (DFB) laser has been used to detect the ErbB2 protein. This DFB laser consists of a polystyrene (PS) film containing a perylenediimide laser dye, deposited over a second-order one dimensional grating fabricated on fused silica by thermal-nanoimprint lithography and subsequent reactive ion etching processes. Specificity of the system to ErbB2 protein biomarker, achieved by functionalizing the PS with anti-ErbB2 monoclonal antibodies, is demonstrated. A concentration limit of detection for ErbB2 protein of 14 ng/mL has been obtained, and no cross-reactivity has been observed with bovine serum albumin (BSA) and tumor necrosis factor alpha (TNFα) proteins. These findings open the possibility of using this type of biosensors in clinical applications. |
Patrocinador/es: | This work was supported by the Spanish Government (MINECO) and the European Community (FEDER) through grant no. MAT-2011–28167-C02. This work was partially funded by the Basque Government within the framework of the Etortek Program (Grant No. IE13-360). M. Morales-Vidal has been partly supported by a MINECO FPI fellowship (no. BES-2009-020747). |
URI: | http://hdl.handle.net/10045/50561 |
ISSN: | 0925-4005 (Print) | 1873-3077 (Online) |
DOI: | 10.1016/j.snb.2015.09.093 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2015 Elsevier B.V. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.snb.2015.09.093 |
Aparece en las colecciones: | INV - Física de la Materia Condensada - Artículos de Revistas INV - GHPO - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2016_Retolaza_etal_SensorsB_final.pdf | Versión final (acceso restringido) | 637,21 kB | Adobe PDF | Abrir Solicitar una copia |
2016_Retolaza_etal_SensorsB_accepted.pdf | Accepted Manuscript (acceso abierto) | 161,32 kB | Adobe PDF | Abrir Vista previa |
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