Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA
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Título: | Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA |
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Autor/es: | Tuells, José | Parra Grande, Mónica | Santos-Calle, Francisco J. | Montagud, Ana C. | Egoavil, Cecilia | García-Rivera, Celia | Caballero, Pablo | Gabaldón Bravo, Eva María | Rodríguez-Díaz, Juan Carlos | Hurtado-Sánchez, José Antonio |
Grupo/s de investigación o GITE: | Salud Comunitaria (SALUD) | Enfermería y Cultura de los Cuidados (EYCC) | Grupo de Investigación en Alimentación y Nutrición (ALINUT) | Psicología Aplicada a la Salud y Comportamiento Humano (PSYBHE) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Enfermería Comunitaria, Medicina Preventiva y Salud Pública e Historia de la Ciencia | Universidad de Alicante. Departamento de Enfermería |
Palabras clave: | Neutralizing antibodies | COVID-19 | SARS-CoV-2 | Serological test | Immunoassay | Lateral flow assay | Lateral flow immunochromatography | Sensitivity and specificity |
Área/s de conocimiento: | Enfermería |
Fecha de publicación: | 25-mar-2022 |
Editor: | MDPI |
Cita bibliográfica: | Tuells J, Parra-Grande M, Santos-Calle FJ, Montagud AC, Egoavil CM, García-Rivera C, Caballero P, Gabaldón-Bravo EM, Rodríguez-Diaz JC, Hurtado-Sánchez JA. Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA. Vaccines. 2022; 10(4):510. https://doi.org/10.3390/vaccines10040510 |
Resumen: | The presence of neutralizing antibodies (NAbs) against SARS-CoV-2 represent a surrogate marker of immunologic protection in populations at high risk of infection such as healthcare workers caring for hospitalized patients with COVID-19. As recommended by CDC and the European CDC, the use of rapid diagnostic tests during population-based evaluations offers an opportunity to identify individuals with serologic evidence of natural infection or who have undergone vaccination. We carried out a cross-sectional study to assess the presence of neutralizing antibodies against SARS-CoV-2 among medical providers at an intensive care unit of a large referral hospital in Alicante, Spain. In addition, we tested for the presence of neutralizing antibodies compared to serum of uninfected individuals from a Biobank. We were also interested in evaluating the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral neutralization test (sVNT). This rapid test demonstrated a specificity of 1.000 95% CI (0.91–1.00) and the sensitivity of 0.987 95% CI (0.93–1.00). The negative predictive value was 95%. After six months, this rapid test demonstrated that those immunized with two doses of BioNTech/Pfizer vaccine, maintained optimal levels of neutralizing antibodies. We concluded that all Health Care Workers develop NAbs and the use of this rapid immunochromatographic test represents a potential tool to be used in population-based studies to detect serological antibody responses to vaccination. Vaccination policies could benefit from this tool to assess additional doses of vaccine or boosters among high-risk populations. |
Patrocinador/es: | This work was supported by an advisory contract from the University of Alicante with reference WENZHOU-OJABIO1-21T and no one reports personal fees received from the biotech company. |
URI: | http://hdl.handle.net/10045/122408 |
ISSN: | 2076-393X |
DOI: | 10.3390/vaccines10040510 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/vaccines10040510 |
Aparece en las colecciones: | INV - SALUD - Artículos de Revistas Cátedra Balmis de Vacunología - Publicaciones INV - PSYBHE - Artículos de Revistas INV - EYCC - Artículos de Revistas INV - ALINUT - Artículos de Revistas |
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