Selection of the N-Acylhomoserine Lactone-Degrading Bacterium Alteromonas stellipolaris PQQ-42 and of Its Potential for Biocontrol in Aquaculture
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Title: | Selection of the N-Acylhomoserine Lactone-Degrading Bacterium Alteromonas stellipolaris PQQ-42 and of Its Potential for Biocontrol in Aquaculture |
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Authors: | Torres, Marta | Rubio-Portillo, Esther | Anton, Josefa | Ramos-Esplá, Alfonso A. | Quesada, Emilia | Llamas, Inmaculada |
Research Group/s: | Ecología Microbiana Molecular | Biología Marina | Recursos Hídricos y Desarrollo Sostenible |
Center, Department or Service: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada |
Keywords: | Quorum quenching | Aquaculture | Vibrio | Biocontrol | N-acylhomoserine lactone |
Knowledge Area: | Microbiología | Zoología |
Issue Date: | 9-May-2016 |
Publisher: | Frontiers Media |
Citation: | Torres M, Rubio-Portillo E, Antón J, Ramos-Esplá AA, Quesada E and Llamas I (2016) Selection of the N-Acylhomoserine Lactone-Degrading Bacterium Alteromonas stellipolaris PQQ-42 and of Its Potential for Biocontrol in Aquaculture. Front. Microbiol. 7:646. doi: 10.3389/fmicb.2016.00646 |
Abstract: | The production of virulence factors by many pathogenic microorganisms depends on the intercellular communication system called quorum sensing, which involves the production and release of signal molecules known as autoinducers. Based on this, new-therapeutic strategies have emerged for the treatment of a variety of infections, such as the enzymatic degradation of signaling molecules, known as quorum quenching (QQ). In this study, we present the screening of QQ activity amongst 450 strains isolated from a bivalve hatchery in Granada (Spain), and the selection of the strain PQQ-42, which degrades a wide range of N-acylhomoserine lactones (AHLs). The selected strain, identified as Alteromonas stellipolaris, degraded the accumulation of AHLs and reduced the production of protease and chitinase and swimming motility of a Vibrio species in co-cultivation experiments in vitro. In the bio-control experiment, strain PQQ-42 significantly reduced the pathogenicity of Vibrio mediterranei VibC-Oc-097 upon the coral Oculina patagonica showing a lower degree of tissue damage (29.25 ± 14.63%) in its presence, compared to when the coral was infected with V. mediterranei VibC-Oc-097 alone (77.53 ± 13.22%). Our results suggest that this AHL-degrading bacterium may have biotechnological applications in aquaculture. |
Sponsor: | This research was supported by grants from the Dirección General de Investigación Científica y Técnica (BIO2011-12879E; AGL2015-68806-R) and from the Plan Andaluz de Investigación (P07-CVI-03150; CVI06226), Spain. MT is supported by a FPU fellowship from the Spanish Ministry of Science and Education (FPU13-0466). |
URI: | http://hdl.handle.net/10045/54866 |
ISSN: | 1664-302X |
DOI: | 10.3389/fmicb.2016.00646 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2016 Torres, Rubio-Portillo, Antón, Ramos-Esplá, Quesada and Llamas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.3389/fmicb.2016.00646 |
Appears in Collections: | INV - BM - Artículos Científicos / Scientific Papers INV - EMM - Artículos de Revistas INV - Recursos Hídricos y Desarrollo Sostenible - Artículos de Revistas |
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