Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa
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http://hdl.handle.net/10045/46906
| Title: | Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa |
|---|---|
| Authors: | Francou, María Manuela | Ten Morro, Jorge | Bernabeu, Rafael | Juan Herrero, Joaquín de |
| Research Group/s: | Biotecnología |
| Center, Department or Service: | Universidad de Alicante. Departamento de Biotecnología |
| Keywords: | Acrosome reaction | Capacitation | Cytoskeleton | Immunocytochemistry | Spermatozoa | Tubulin |
| Knowledge Area: | Biología Celular |
| Issue Date: | Feb-2014 |
| Publisher: | Elsevier |
| Citation: | Reproductive BioMedicine Online. 2014, 28(2): 246-250. doi:10.1016/j.rbmo.2013.10.001 |
| Abstract: | Tubulin is a protein constituent of cytoskeletal microtubules, closely related to sperm motility. However, the changes in tubulin distribution following capacitation and acrosome reaction are poorly understood. This study immunolocalized and quantified the expression of α-tubulin in fresh, capacitated and acrosome-reacted samples. Immunocytochemical data showed that in capacitated and acrosome-reacted spermatozoa, α-tubulin is labelled throughout most of the flagellum (⩾66.66%). However, the mean α-tubulin-labelled area in these samples was significantly lower than in fresh samples (P < 0.05). Thus, there are different sperm clusters distinguished by their α-tubulin immunoreactivity and this could be directly linked to structural changes following capacitation and acrosome reaction. |
| Sponsor: | This project was supported by the Cátedra de Medicina de la Reproducción, Alicante University-Instituto Bernabeu, Grant 4–12I to JDJ, as well as the Vicerrectorado de Innovación, University of Alicante, Spain, Grant Vigrob-137 to JDJ. |
| URI: | http://hdl.handle.net/10045/46906 |
| ISSN: | 1472-6483 (Print) | 1472-6491 (Online) |
| DOI: | 10.1016/j.rbmo.2013.10.001 |
| Language: | eng |
| Type: | info:eu-repo/semantics/article |
| Rights: | © 2013, Reproductive Healthcare Ltd. Published by Elsevier Ltd. |
| Peer Review: | si |
| Publisher version: | http://dx.doi.org/10.1016/j.rbmo.2013.10.001 |
| Appears in Collections: | INV - GIDBT - Artículos de Revistas Institucional - IUIEG - Publicaciones |
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| File | Description | Size | Format | |
|---|---|---|---|---|
 2014_Francou_etal_RBM-online_final.pdf | Versión final (acceso restringido) | 998,43 kB | Adobe PDF | Open Request a copy |
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