Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm
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http://hdl.handle.net/10045/125071
Títol: | Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm |
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Autors: | Gómez-Torres, María José | Huerta-Retamal, Natalia | Sáez Espinosa, Paula | Robles-Gómez, Laura | Avilés Sánchez, Manuel | Aizpurua, Jon |
Grups d'investigació o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Biotecnología |
Paraules clau: | Heat shock protein | HSP70 | Immunofluorescence | Male infertility | Sperm selection |
Àrees de coneixement: | Biología Celular |
Data de publicació: | 11-de juliol-2022 |
Editor: | Springer Nature |
Citació bibliogràfica: | Reproductive Sciences. 2023, 30: 1176-1185. https://doi.org/10.1007/s43032-022-01031-9 |
Resum: | During fertilization, sperm hyaluronidase activity is essential for spermatozoa to successfully penetrate the hyaluronic acid-enriched extracellular matrix of the cumulus cells. Since molecular chaperones, as the heat shock protein A2, are typically involved in bringing hyaluronic acid receptors to the cell surface, here we evaluated the presence and spatial location of HSPA2 on human spermatozoa based on its hyaluronic acid binding capacity. This study included 16 normozoospermic sperm samples from volunteering donors. The location of HSPA2 was studied in cells before and after 1-h incubation under capacitating conditions, as well as in spermatozoa selected according to their ability of binding to hyaluronic acid. Our results showed no significant differences in HSPA2 immunofluorescent cells before and after 1 h of incubation in capacitating conditions. Nevertheless, after hyaluronic acid selection, the percentage of HSPA2-labelled cells increased significantly, indicating that the interaction with hyaluronic acid may induce the unmasking of HSPA2 epitopes. Furthermore, after swim-up and hyaluronic acid selection, spermatozoa presented a highly immunostained equatorial band with a homogeneous fluorescence throughout the acrosomal region. This distribution has been previously suggested to have important implications in male fertility. Noteworthy, a homogeneous fluorescence among the acrosomal region with a more intense labelling at the apical region was observed only in hyaluronic acid bound sperm cells, which may be associated with primary gamete recognition. Our findings suggest that the hyaluronic acid selection technique and HSPA2 biomarker should be considered candidates to complement the classic seminal analysis before recommending an appropriate assisted reproduction technique. |
Patrocinadors: | Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by the Human Fertility Cathedra of the University of Alicante and R&D&I projects financed by competitive public entities (ViGrob-186, UAIND17-03, PGC2018-094781-B-100). |
URI: | http://hdl.handle.net/10045/125071 |
ISSN: | 1933-7205 |
DOI: | 10.1007/s43032-022-01031-9 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Revisió científica: | si |
Versió de l'editor: | https://doi.org/10.1007/s43032-022-01031-9 |
Apareix a la col·lecció: | INV - Grupo de Inmunología - Artículos de Revistas |
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Gomez-Torres_etal_2023_ReprodSci.pdf | 1,19 MB | Adobe PDF | Obrir Vista prèvia | |
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