Functional analysis of Lsm protein under multiple stress conditions in the extreme haloarchaeon Haloferax mediterranei
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http://hdl.handle.net/10045/115052
Títol: | Functional analysis of Lsm protein under multiple stress conditions in the extreme haloarchaeon Haloferax mediterranei |
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Autors: | Payá, Gloria | Bautista, Vanesa | Camacho, Mónica | Bonete, María-José | Esclapez, Julia |
Grups d'investigació o GITE: | Biotecnología de Extremófilos (BIOTECEXTREM) |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Paraules clau: | Skm gene | Haloarchaea | Deletion mutant | RT-PCR | Stress assays |
Àrees de coneixement: | Bioquímica y Biología Molecular |
Data de publicació: | 13-de maig-2021 |
Editor: | Elsevier |
Citació bibliogràfica: | Biochimie. 2021, 187: 33-47. https://doi.org/10.1016/j.biochi.2021.05.002 |
Resum: | The Sm, like-Sm, and Hfq proteins belonging to the Sm superfamily of proteins are represented in all domains of life. These proteins are involved in several RNA metabolism pathways. The functions of bacterial Hfq and eukaryotic Sm proteins have been described, but knowledge about the in vivo functions of archaeal Sm proteins remains limited. This study aims to improve the understanding of Lsm proteins and their role using the haloarchaeon Haloferax mediterranei as a model microorganism. The Haloferax mediterranei genome contains one lsm gene that overlaps with the rpl37e gene. To determine the expression of lsm and rpl37e genes and the co-transcription of both, reverse transcription-polymerase chain reaction (RT-PCR) analyses were performed under different standard and stress conditions. The results suggest that the expression of lsm and rpl37e is constitutive. Co-transcription occurs at sub-optimal salt concentrations and temperatures, depending on the growth phase. The halophilic Lsm protein contains two Sm motifs, Sm1 and Sm2, and the sequence encoding the Sm2 motif also constitutes the promoter of the rpl37e gene. To investigate their biological functions, the lsm deletion mutant and the Sm1 motif deletion mutant, where the Sm2 motif remained intact, were generated and characterised. Comparison of the lsm deletion mutant, Sm1 deletion mutant, and the parental strain HM26 under standard and stress growth conditions revealed growth differences. Finally, swarming assays in complex and defined media showed greater swarming capacity in the deletion mutants. |
Patrocinadors: | This work was supported by the Spanish Ministry of Economy and Competitiveness [grant BIO2013-42921-P]; Programa Propio para el fomento de la I+D+I del Vicerrectorado de Investigación y Transferencia de Conocimiento” of the University of Alicante (VIGRO-016) and the Generalitat Valenciana (Spain) [ACIF/2018/200]. |
URI: | http://hdl.handle.net/10045/115052 |
ISSN: | 0300-9084 (Print) | 1638-6183 (Online) |
DOI: | 10.1016/j.biochi.2021.05.002 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Revisió científica: | si |
Versió de l'editor: | https://doi.org/10.1016/j.biochi.2021.05.002 |
Apareix a la col·lecció: | INV - BIOTECEXTREM - Artículos de Revistas |
Arxius per aquest ítem:
Arxiu | Descripció | Tamany | Format | |
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Paya_etal_2021_Biochimie_final.pdf | 3,49 MB | Adobe PDF | Obrir Vista prèvia | |
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