Environmental dissolved DNA harbours meaningful biological information on microbial community structure
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http://hdl.handle.net/10045/114641
Title: | Environmental dissolved DNA harbours meaningful biological information on microbial community structure |
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Authors: | Aldeguer-Riquelme, Borja | Ramos-Barbero, María Dolores | Santos, Fernando | Anton, Josefa |
Research Group/s: | Ecología Microbiana Molecular |
Center, Department or Service: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Keywords: | eDNA | dDNA | Hypersaline | Nanohaloarchaeota | Nanohalovirus | Haloquadratum | Halovirus |
Knowledge Area: | Microbiología |
Issue Date: | 5-Apr-2021 |
Publisher: | Society for Applied Microbiology | John Wiley & Sons |
Citation: | Environmental Microbiology. 2021, 23(5): 2669-2682. https://doi.org/10.1111/1462-2920.15510 |
Abstract: | Extracellular DNA (eDNA) comprises all the DNA molecules outside cells. This component of microbial ecosystems may serve as a source of nutrients and genetic information. Hypersaline environments harbour one of the highest concentrations of eDNA reported for natural systems, which has been attributed to the physicochemical preservative effect of salts and to high viral abundance. Here, we compared centrifugation and filtration protocols for the extraction of dissolved DNA (dDNA, as opposed to eDNA that also includes DNA from free viral particles) from a solar saltern crystallizer pond (CR30) water sample. The crystallizer dDNA fraction has been characterized, for the first time, and compared with cellular and viral metagenomes from the same location. High‐speed centrifugation affected CR30 dDNA concentration and composition due to cell lysis, highlighting that protocol optimization should be the first step in dDNA studies. Crystallizer dDNA, which accounted for lower concentrations than those previously reported for hypersaline anoxic sediments, had a mixed viral and cellular origin, was enriched in archaeal DNA and had a distinctive taxonomic composition compared to that from the cellular assemblage of the same sample. Bioinformatic analyses indicated that nanohaloarchaeal viruses could be a cause for these differences. |
Sponsor: | This research was supported by the Spanish Ministry of Science, Innovation and Universities grant MICROMATES (PGC2018-096956-B-C44), which was also supported with European Regional Development Fund (FEDER) funds, and by the Generalitat Valenciana grant PROMETEO/2017/129. B.A.-R. is a ACIF (Generalitat Valenciana) fellow. |
URI: | http://hdl.handle.net/10045/114641 |
ISSN: | 1462-2912 (Print) | 1462-2920 (Online) |
DOI: | 10.1111/1462-2920.15510 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2021 Society for Applied Microbiology and John Wiley & Sons Ltd. |
Peer Review: | si |
Publisher version: | https://doi.org/10.1111/1462-2920.15510 |
Appears in Collections: | INV - EMM - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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Aldeguer-Riquelme_etal_2021_EnvironMicrobiol_accepted.pdf | Accepted Manuscript (acceso abierto) | 4,14 MB | Adobe PDF | Open Preview |
Aldeguer-Riquelme_etal_2021_EnvironMicrobiol_final.pdf | Versión final (acceso restringido) | 2,4 MB | Adobe PDF | Open Request a copy |
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