Haloferax mediterranei Cells as C50 Carotenoid Factories
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Title: | Haloferax mediterranei Cells as C50 Carotenoid Factories |
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Authors: | Giani, Micaela | Montero-Lobato, Zaida | Garbayo, Inés | Vílchez, Carlos | Vega, José María | Martínez-Espinosa, Rosa María |
Research Group/s: | Bioquímica Aplicada/Applied Biochemistry (AppBiochem) |
Center, Department or Service: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Keywords: | Haloferax mediterranei | Bacterioruberin | Carotenoids | C/N ratio | Osmotic stress |
Knowledge Area: | Bioquímica y Biología Molecular |
Issue Date: | 10-Feb-2021 |
Publisher: | MDPI |
Citation: | Giani M, Montero-Lobato Z, Garbayo I, Vílchez C, Vega JM, Martínez-Espinosa RM. Haloferax mediterranei Cells as C50 Carotenoid Factories. Marine Drugs. 2021; 19(2):100. https://doi.org/10.3390/md19020100 |
Abstract: | Haloarchaea produce C50 carotenoids such as bacterioruberin, which are of biotechnological in-terest. This study aimed to analyze the effect of different environmental and nutritional conditions on the cellular growth and dynamics of carotenoids accumulation in Haloferax mediterranei. The maximum production of carotenoids (40 µg·mL−1) was obtained during the stationary phase of growth, probably due to nutrient-limiting conditions (one-step culture). By seven days of culture, 1 mL culture produced 22.4 mg of dry weight biomass containing 0.18 % (w/w) of carotenoids. On the other hand, carbon-deficient cultures (low C/N ratio) were observed to be optimum for C50 bacterioruberin production by Hfx. mediterranei, but negatively affected the growth of cells. Thus, a two-steps process was evaluated for optimum carotenoids yield. In the first step, a nutri-ent-repleted culture medium enabled the haloarchaea to produce biomass, while in the second step, the biomass was incubated under osmotic stress and in a carbon-deficient medium. Under the conditions used, the obtained biomass contained 0.27% (w/w) of carotenoids after seven days, which accounts for 58.49 µg·mL−1 of carotenoids for a culture with turbidity 14.0. |
Sponsor: | This work was funded by a research grant from MINECO Spain (RTI2018-099860-B-I00) and the University of Alicante (VIGROB-309). We are also indebted to the Andalusian Government (research project BIO-214). ZM was assisted by a pre-doctoral grant from “Plan Propio de Investigación” of the University of Huelva, Spain. MG was awarded with a pre-doctoral fellowship from the Valencian Community Government (ACIF/2019/043). |
URI: | http://hdl.handle.net/10045/112904 |
ISSN: | 1660-3397 |
DOI: | 10.3390/md19020100 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
Peer Review: | si |
Publisher version: | https://doi.org/10.3390/md19020100 |
Appears in Collections: | INV - AppBiochem - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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Giani_etal_2021_MarDrugs.pdf | 1,38 MB | Adobe PDF | Open Preview | |
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