Elexpuru Artetxe, Ana, Martín-Nieto, José, Jiménez Martínez, Amparo, Gómez Torres, Carmen, Villalobo Polo, Antonio
Ehrlich ascites tumor cells produce a transforming growth factor-β (TGFβ)-like activity but lack receptors with TGFβ-binding capacity
ELEXPURU ARTETXE, Ana, et al. "Ehrlich ascites tumor cells produce a transforming growth factor-β (TGFβ)-like activity but lack receptors with TGFβ-binding capacity". Molecular and Cellular Biochemistry. Vol. 170, No. 1-2 (May 1997). ISSN 0300-8177, pp. 153-162
URI: http://hdl.handle.net/10045/9728
DOI: 10.1023/A:1006809604193
ISSN: 0300-8177 (Print)
Abstract: 
Ehrlich ascites tumor cells incorporate [methyl-3H]thymidine into DNA independently of exogenous growth factors or fetal
calf serum. Using an acid/ethanol extraction procedure we have obtained from these tumor cells a fraction that induces both
the proliferation and the formation of cell foci by Swiss 3T3 mouse fibroblasts in the presence of insulin; inhibits the proliferation of Mv1Lu mink lung epithelial cells; and stimulates the growth of NRK rat kidney fibroblasts in soft-agar in the presence of epidermal growth factor. An antibody against transforming growth factor-β (TGFβ) prevents both the tumor extract-induced proliferation of Swiss 3T3 fibroblasts and the tumor extract-induced proliferative arrest of Mv1Lu cells. The tumor cells secrete a TGFβ-like activity to the extracellular medium in a partially-activated form. However, authentic TGFβ does not affect their proliferation, and no TGFβ receptors were detected using [125I]TGFβ as a ligand. Therefore, the absence of TGFβ receptors with ligand-binding capacity in these tumor cells may bypass the negative control that this factor exerts on the proliferation of their normal cell counterparts.
Keywords:Transforming growth factor-β receptors, Ehrlich ascites tumor cells
Kluwer Academic Publishers
info:eu-repo/semantics/article