Čičková, Helena, Pastor Monllor, Berta, Kozánek, Milan, Martínez-Sánchez, Anabel, Rojo, Santos, Takáč, Peter Biodegradation of pig manure by the housefly, Musca domestica: a viable ecological strategy for pig manure management ČIČKOVÁ, Helena, et al. “Biodegradation of pig manure by the housefly, Musca domestica: a viable ecological strategy for pig manure management”. PLoS ONE 7(3): e32798. doi:10.1371/journal.pone.0032798 URI: http://hdl.handle.net/10045/33416 DOI: 10.1371/journal.pone.0032798 ISSN: 1932-6203 Abstract: The technology for biodegradation of pig manure by using houseflies in a pilot plant capable of processing 500–700 kg of pig manure per week is described. A single adult cage loaded with 25,000 pupae produced 177.7±32.0 ml of eggs in a 15-day egg-collection period. With an inoculation ratio of 0.4–1.0 ml eggs/kg of manure, the amount of eggs produced by a single cage can suffice for the biodegradation of 178–444 kg of manure. Larval development varied among four different types of pig manure (centrifuged slurry, fresh manure, manure with sawdust, manure without sawdust). Larval survival ranged from 46.9±2.1%, in manure without sawdust, to 76.8±11.9% in centrifuged slurry. Larval development took 6–11 days, depending on the manure type. Processing of 1 kg of wet manure produced 43.9–74.3 g of housefly pupae and the weight of the residue after biodegradation decreased to 0.18–0.65 kg, with marked differences among manure types. Recommendations for the operation of industrial-scale biodegradation facilities are presented and discussed. Keywords:Biodegradation, Pig manure, Houseflies Public Library of Science (PLoS) info:eu-repo/semantics/article