Prokaryotic and viral community of the sulfate‐rich crust from Peñahueca ephemeral lake, an astrobiology analogue
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Título: | Prokaryotic and viral community of the sulfate‐rich crust from Peñahueca ephemeral lake, an astrobiology analogue |
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Autor/es: | Martín Cuadrado, Ana Belén | Senel, Ece | Martinez-Garcia, Manuel | Cifuentes, Ana | Santos, Fernando | Almansa Carrascosa, María Cristina | Moreno-Paz, Mercedes | Blanco, Yolanda | García-Villadangos, Miriam | García del Cura, María Ángeles | Sanz Montero, María Esther | Rodríguez-Aranda, Juan Pablo | Rosselló Móra, Ramon | Anton, Josefa | Parro, Víctor |
Grupo/s de investigación o GITE: | Ecología Microbiana Molecular |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Servicios Técnicos de Investigación |
Palabras clave: | Hypersaline sulfate-rich crust | Athalassohaline | Halophilic | 16S rRNA | Metagenome | Metavirome | CRISPR |
Área/s de conocimiento: | Microbiología |
Fecha de publicación: | oct-2019 |
Editor: | Wiley |
Cita bibliográfica: | Environmental Microbiology. 2019, 21(10): 3577-3600. doi:10.1111/1462-2920.14680 |
Resumen: | Peñahueca is an athalassohaline hypersaline inland ephemeral lake originated under semiarid conditions in the central Iberian Peninsula (Spain). Its chemical composition makes it extreme for microbial life as well as a terrestrial analogue of other planetary environments. To investigate the persistence of microbial life associated with sulfate‐rich crusts, we applied cultivation‐independent methods (optical and electron microscopy, 16S rRNA gene profiling and metagenomics) to describe the prokaryotic community and its associated viruses. The diversity for Bacteria was very low and was vastly dominated by endospore formers related to Pontibacillus marinus of the Firmicutes phylum. The archaeal assemblage was more diverse and included taxa related to those normally found in hypersaline environments. Several ‘metagenome assembled genomes’ were recovered, corresponding to new species of Pontibacillus, several species from the Halobacteria and one new member of the Nanohaloarchaeota. The viral assemblage, although composed of the morphotypes typical of high salt systems, showed little similarity to previously isolated/reconstructed halophages. Several putative prophages of Pontibacillus and haloarchaeal hosts were identified. Remarkably, the Peñahueca sulfate‐rich metagenome contained CRISPR‐associated proteins and repetitions which were over 10‐fold higher than in most hypersaline systems analysed so far. |
Patrocinador/es: | This research was supported by the Spanish Ministry of Economy projects CLG2015_66686-C3-1 (to RRM) CLG2015_66686-C3-3 (to JA), CGL2015-66455-R (to MAGC, MESM, JPRA), AYA2011-24803 and ESP2015-69540-R (to VP) which were also supported by the European Regional Development Fund and the MDM-2017-0737 Unidad de Excelencia “María de Maeztu”- Centro de Astrobiología (INTA-CSIC). |
URI: | http://hdl.handle.net/10045/97170 |
ISSN: | 1462-2912 (Print) | 1462-2920 (Online) |
DOI: | 10.1111/1462-2920.14680 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2019 Society for Applied Microbiology and John Wiley & Sons Ltd. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1111/1462-2920.14680 |
Aparece en las colecciones: | INV - EMM - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2019_Martin-Cuadrado_etal_EnvironMicrobiol_final.pdf | Versión final (acceso restringido) | 3,1 MB | Adobe PDF | Abrir Solicitar una copia |
2019_Martin-Cuadrado_etal_EnvironMicrobiol_accepted.pdf | Accepted Manuscript (acceso abierto) | 890,94 kB | Adobe PDF | Abrir Vista previa |
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