Cortistatin regulates glucose-induced electrical activity and insulin secretion in mouse pancreatic beta-cells
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Título: | Cortistatin regulates glucose-induced electrical activity and insulin secretion in mouse pancreatic beta-cells |
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Autor/es: | Soriano, Sergi | Castellano-Muñoz, Manuel | Rafacho, Alex | Alonso Magdalena, Paloma | Marroquí, Laura | Ruiz, Antonia | Bru-Tarí, Eva | Merino, Beatriz | Irles, Esperanza | Bello, Melisa | Iborra, Pau | Villar-Pazos, Sabrina | Vettorazzi, Jean Franciesco | Montanya, Eduard | Luque, Raúl M. | Nadal, Ángel | Quesada, Iván |
Grupo/s de investigación o GITE: | Fisiología Neuroendocrina (FINE) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Pancreatic beta-cell | Insulin secretion | Cortistatin | KATP channels | GIRK channels |
Área/s de conocimiento: | Fisiología |
Fecha de publicación: | 24-sep-2018 |
Editor: | Elsevier |
Cita bibliográfica: | Molecular and Cellular Endocrinology. 2019, 479: 123-132. doi:10.1016/j.mce.2018.09.009 |
Resumen: | Although there is growing evidence that cortistatin regulates several functions in different tissues, its role in the endocrine pancreas is not totally known. Here, we aim to study the effect of cortistatin on pancreatic beta-cells and glucose-stimulated insulin secretion (GSIS). Exposure of isolated mouse islets to cortistatin inhibited GSIS. This effect was prevented using a somatostatin receptor antagonist. Additionally, cortistatin hyperpolarized the membrane potential and reduced glucose-induced action potentials in isolated pancreatic beta-cells. Cortistatin did not modify ATP-dependent K+ (KATP) channel activity. In contrast, cortistatin increased the activity of a small conductance channel with characteristics of G protein-coupled inwardly rectifying K+ (GIRK) channels. The cortistatin effects on membrane potential and GSIS were largely reduced in the presence of a GIRK channel antagonist and by down-regulation of GIRK2 with small interfering RNA. Thus, cortistatin acts as an inhibitory signal for glucose-induced electrical activity and insulin secretion in the mouse pancreatic beta-cell. |
Patrocinador/es: | This work was supported by grants from the Ministerio de Economía, Industria y Competitividad (Spain), Agencia Estatal de Investigación (Spain) and Fondo Europeo de Desarrollo Regional (BFU2013-42789; BFU2017-86579-R; BFU2016-77125-R), Generalitat Valenciana (Spain, PROMETEOII/2015/016), CNPq (Brazil; 302261/2014-1; 306359/2017-0) and Junta de Andalucía (Spain; CTS-1406, BIO-0139). L.M. holds a Juan de la Cierva fellowship from the Ministry of Economy, Industry and Competitiveness (Spain; IJCI-2015-24482). CIBERDEM and CIBERobn are an initiative of the Instituto de Salud Carlos III (Spain). |
URI: | http://hdl.handle.net/10045/82807 |
ISSN: | 0303-7207 (Print) | 1872-8057 (Online) |
DOI: | 10.1016/j.mce.2018.09.009 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2018 Elsevier B.V. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.mce.2018.09.009 |
Aparece en las colecciones: | INV - FINE - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2018_Soriano_etal_MolCelEndocrin_accepted.pdf | Embargo 12 meses (acceso abierto: 25 sept. 2019) | 1,2 MB | Adobe PDF | Abrir Solicitar una copia |
2018_Soriano_etal_MolCelEndocrin_final.pdf | Versión final (acceso restringido) | 1,63 MB | Adobe PDF | Abrir Solicitar una copia |
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