Genes related to cell-mediated cytotoxicity and interferon response are induced in the retina of European sea bass upon intravitreal infection with nodavirus
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Título: | Genes related to cell-mediated cytotoxicity and interferon response are induced in the retina of European sea bass upon intravitreal infection with nodavirus |
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Autor/es: | Valero, Yulema | Boughlala, Bassima | Arizcun, Marta | Patel, Sonal | Fiksdal, Ingrid U. | Esteban, M. Ángeles | Juan Herrero, Joaquín de | Meseguer, José | Chaves-Pozo, Elena | Cuesta, Alberto |
Grupo/s de investigación o GITE: | Biotecnología |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | Nodavirus | European sea bass | Retina | Innate immune response | Adaptive immune response | Interferon | Cytotoxicity |
Área/s de conocimiento: | Biología Celular |
Fecha de publicación: | mar-2018 |
Editor: | Elsevier |
Cita bibliográfica: | Fish & Shellfish Immunology. 2018, 74: 627-636. doi:10.1016/j.fsi.2018.01.034 |
Resumen: | Viral diseases are responsible for high rates of mortality and subsequent economic losses in modern aquaculture. The nervous necrosis virus (NNV) produces viral encephalopathy and retinopathy (VER), which affects the central nervous system, is considered one of the most serious viral diseases in marine aquaculture. Although some studies have localized NNV in the retina cells, none has dealt with immunity in the retina. Thus, for the first time, we intravitreally infected healthy specimens of European sea bass (Dicentrarchus labrax) with NNV with the aim of characterizing the immune response in the retina. Ultrastructural analysis detected important retinal injuries and structure degradation, including pycnosis, hydropic degeneration and vacuolization in some cell layers as well as myelin sheaths in the optic nerve fibres. Immunohistochemistry demonstrated that NNV replicated in the eyes. Regarding retinal immunity, NNV infection elicited the transcription of genes encoding proteins involved in the interferon (IFN) and cell-mediated cytotoxicity (CMC) responses as well as B and T cell markers, demonstrating that viral replication influences innate and adaptive responses. Further studies are needed to understand the retina immunity and whether the main retinal function, vision, is affected by nodavirus. |
Patrocinador/es: | This work was supported by grants of the Ministerio de Economía y Competitividad and FEDER (AGL2013-43588-P), Fundacion Séneca (Grupo de Excelencia de la Region de Murcia19883/GERM/15), Instituto Español de Oceanografía (NODAMED), and European Union Seventh Framework Programme for research, technological development and demonstration (KBBE-2013-07 single stage, GA 603121, DIVERSIFY). Yulema Valero thanks the Instituto Español de Ocenaografía for her PhD grant. Nodavirus strain and SSN-1 cells were kindly donated by Pilar Fernández Somalo (Laboratorio Central de Veterinaria de Algete, Ministerio de Medio Ambiente, Rural y Marino). |
URI: | http://hdl.handle.net/10045/77033 |
ISSN: | 1050-4648 (Print) | 1095-9947 (Online) |
DOI: | 10.1016/j.fsi.2018.01.034 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2018 Elsevier Ltd. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.fsi.2018.01.034 |
Aparece en las colecciones: | Investigaciones financiadas por la UE INV - GIDBT - Artículos de Revistas |
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2018_Valero_etal_FishShellfishImmuno_final.pdf | Versión final (acceso restringido) | 1,15 MB | Adobe PDF | Abrir Solicitar una copia |
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