A Calcium-Dependent Chloride Current Increases Repetitive Firing in Mouse Sympathetic Neurons
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Título: | A Calcium-Dependent Chloride Current Increases Repetitive Firing in Mouse Sympathetic Neurons |
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Autor/es: | Martinez-Pinna, Juan | Soriano, Sergi | Tudurí, Eva | Nadal, Ángel | Castro, Fernando de |
Grupo/s de investigación o GITE: | Fisiología Neuroendocrina (FINE) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Sympathetic neuron | Chloride current | Repetitive firing | Gender differences | Anthracene-9′-carboxylic acid |
Área/s de conocimiento: | Fisiología |
Fecha de publicación: | 14-may-2018 |
Editor: | Frontiers Media |
Cita bibliográfica: | Martinez-Pinna J, Soriano S, Tudurí E, Nadal A and de Castro F (2018) A Calcium-Dependent Chloride Current Increases Repetitive Firing in Mouse Sympathetic Neurons. Front. Physiol. 9:508. doi: 10.3389/fphys.2018.00508 |
Resumen: | Ca2+-activated ion channels shape membrane excitability in response to elevations in intracellular Ca2+. The most extensively studied Ca2+-sensitive ion channels are Ca2+-activated K+ channels, whereas the physiological importance of Ca2+-activated Cl- channels has been poorly studied. Here we show that a Ca2+-activated Cl- currents (CaCCs) modulate repetitive firing in mouse sympathetic ganglion cells. Electrophysiological recording of mouse sympathetic neurons in an in vitro preparation of the superior cervical ganglion (SCG) identifies neurons with two different firing patterns in response to long depolarizing current pulses (1 s). Neurons classified as phasic (Ph) made up 67% of the cell population whilst the remainders were tonic (T). When a high frequency train of spikes was induced by intracellular current injection, SCG sympathetic neurons reached an afterpotential mainly dependent on the ratio of activation of two Ca2+-dependent currents: the K+ [IK(Ca)] and CaCC. When the IK(Ca) was larger, an afterhyperpolarization was the predominant afterpotential but when the CaCC was larger, an afterdepolarization (ADP) was predominant. These afterpotentials can be observed after a single action potential (AP). Ph and T neurons had similar ADPs and hence, the CaCC does not seem to determine the firing pattern (Ph or T) of these neurons. However, inhibition of Ca2+-activated Cl- channels with anthracene-9′-carboxylic acid (9AC) selectively inhibits the ADP, reducing the firing frequency and the instantaneous frequency without affecting the characteristics of single- or first-spike firing of both Ph and T neurons. Furthermore, we found that the CaCC underlying the ADP was significantly larger in SCG neurons from males than from females. Furthermore, the CaCC ANO1/TMEM16A was more strongly expressed in male than in female SCGs. Blocking ADPs with 9AC did not modify synaptic transmission in either Ph or T neurons. We conclude that the CaCC responsible for ADPs increases repetitive firing in both Ph and T neurons, and it is more relevant in male mouse sympathetic ganglion neurons. |
Patrocinador/es: | This work was supported by grants PB92-0347 and PM95-0107 (from the Dirección General de Investigación Científica y Técnica, Spain) to Roberto Gallego and the Instituto de Cultura Juan Gil-Albert (Diputación de Alicante, Spain) to FdC. Our current work was supported by grants SAF2016-77575-R and RD16/0015/0019 (both from Ministerio de Economía, Innovación y Competitividad-MINEICO, Spain) to FdC and Generalitat Valenciana, PROMETEOII/2015/016 to AN. CIBERDEM is an initiative of the Instituto de Salud Carlos III. |
URI: | http://hdl.handle.net/10045/75508 |
ISSN: | 1664-042X |
DOI: | 10.3389/fphys.2018.00508 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2018 Martinez-Pinna, Soriano, Tudurí, Nadal and de Castro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3389/fphys.2018.00508 |
Aparece en las colecciones: | INV - FINE - Artículos de Revistas INV - NEUROVIS - Artículos de Revistas |
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