Cell wall composition plays a key role on sensitivity of filamentous fungi to chitosan
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Title: | Cell wall composition plays a key role on sensitivity of filamentous fungi to chitosan |
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Authors: | Aranda-Martínez, Almudena | Lopez-Moya, Federico | Lopez-Llorca, Luis Vicente |
Research Group/s: | Fitopatología |
Center, Department or Service: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Keywords: | Neurospora crassa | Pochonia chlamydosporia | Caspofungin | Chitosan | Antifungal |
Knowledge Area: | Botánica |
Issue Date: | Oct-2016 |
Publisher: | Wiley-VCH Verlag GmbH & Co. KGaA |
Citation: | Journal of Basic Microbiology. 2016, 56(10): 1059-1070. doi:10.1002/jobm.201500775 |
Abstract: | Chitosan antifungal activity has been reported for both filamentous fungi and yeast. Previous studies have shown fungal plasma membrane as main chitosan target. However, the role of the fungal cell wall (CW) in their response to chitosan is unknown. We show that cell wall regeneration in Neurospora crassa (chitosan sensitive) protoplasts protects them from chitosan damage. Caspofungin, a β-1,3-glucan synthase inhibitor, showed a synergistic antifungal effect with chitosan for N. crassa but not for Pochonia chlamydosporia, a biocontrol fungus resistant to chitosan. Chitosan significantly repressed N. crassa genes involved in β-1,3-glucan synthesis (fks) and elongation (gel-1) but the chitin synthase gene (chs-1) did not present changes in its expression. N. crassa cell wall deletion strains related to β-1,3-glucan elongation (Δgel-1 and Δgel-2) were more sensitive to chitosan than wild type (wt). On the contrary, chitin synthase deletion strain (Δchs-1) showed the same sensitivity to chitosan than wt. The mycelium of P. chlamydosporia showed a higher (ca. twofold) β-1,3-glucan/chitin ratio than that of N. crassa. Taken together, our results indicate that cell wall composition plays an important role on sensitivity of filamentous fungi to chitosan. |
Sponsor: | This research was funded by the Spanish Ministry of Economy and Competitiveness Grant AGL2015-66833-R and with a grant from the Generalitat Valenciana to A. Aranda-Martinez (ACIF/2013/120). |
URI: | http://hdl.handle.net/10045/62032 |
ISSN: | 0233-111X (Print) | 1521-4028 (Online) |
DOI: | 10.1002/jobm.201500775 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2016 WILEY-VCH Verlag GmbH & Co.KGaA,Weinheim |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.1002/jobm.201500775 |
Appears in Collections: | INV - Fitopatología - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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2016_Aranda_etal_JBasicMicrobiol_final.pdf | Versión final (acceso restringido) | 1,11 MB | Adobe PDF | Open Request a copy |
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