Carbon and nitrogen limitation increase chitosan antifungal activity in Neurospora crassa and fungal human pathogens
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Título: | Carbon and nitrogen limitation increase chitosan antifungal activity in Neurospora crassa and fungal human pathogens |
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Autor/es: | Lopez-Moya, Federico | Colom-Valiente, Maria F. | Martínez-Peinado, Pascual | Martinez-Lopez, Jesus E. | Puelles, Eduardo | Sempere Ortells, José Miguel | Lopez-Llorca, Luis Vicente |
Grupo/s de investigación o GITE: | Fitopatología | Grupo de Inmunología |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Departamento de Biotecnología | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Palabras clave: | Candida spp. | Fusarium proliferatum | Mammalian cell lines | Membrane permeabilization | Nutrient limitation | ROS |
Área/s de conocimiento: | Botánica | Inmunología |
Fecha de publicación: | mar-2015 |
Editor: | Elsevier |
Cita bibliográfica: | Fungal Biology. 2015, 119(2-3): 154-169. doi:10.1016/j.funbio.2014.12.003 |
Resumen: | Chitosan permeabilizes plasma membrane and kills sensitive filamentous fungi and yeast. Membrane fluidity and cell energy determine chitosan sensitivity in fungi. A five-fold reduction of both glucose (main carbon (C) source) and nitrogen (N) increased 2-fold Neurospora crassa sensitivity to chitosan. We linked this increase with production of intracellular reactive oxygen species (ROS) and plasma membrane permeabilization. Releasing N. crassa from nutrient limitation reduced chitosan antifungal activity in spite of high ROS intracellular levels. With lactate instead of glucose, C and N limitation increased N. crassa sensitivity to chitosan further (4-fold) than what glucose did. Nutrient limitation also increased sensitivity of filamentous fungi and yeast human pathogens to chitosan. For Fusarium proliferatum, lowering 100-fold C and N content in the growth medium, increased 16-fold chitosan sensitivity. Similar results were found for Candida spp. (including fluconazole resistant strains) and Cryptococcus spp. Severe C and N limitation increased chitosan antifungal activity for all pathogens tested. Chitosan at 100 μg ml-1 was lethal for most fungal human pathogens tested but non-toxic to HEK293 and COS7 mammalian cell lines. Besides, chitosan increased 90% survival of Galleria mellonella larvae infected with C. albicans. These results are of paramount for developing chitosan as antifungal. |
Patrocinador/es: | This work was supported by Grants from the Spanish Ministries of Economy and Competitiveness (AGL 2011-29297/AGR) and (BFU 2010-16548). |
URI: | http://hdl.handle.net/10045/53264 |
ISSN: | 1878-6146 |
DOI: | 10.1016/j.funbio.2014.12.003 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2014 The British Mycological Society. Published by Elsevier Ltd. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.funbio.2014.12.003 |
Aparece en las colecciones: | INV - Fitopatología - Artículos de Revistas INV - Grupo de Inmunología - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2015_Lopez-Moya_etal_FunBio_final.pdf | Versión final (acceso restringido) | 2,93 MB | Adobe PDF | Abrir Solicitar una copia |
2015_Lopez-Moya_etal_FunBio_accepted.pdf | Accepted Manuscript (acceso abierto) | 22,62 MB | Adobe PDF | Abrir Vista previa |
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