Electrochemical Devices for Monitoring Biomarkers in Embryo Development
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http://hdl.handle.net/10045/47220
Títol: | Electrochemical Devices for Monitoring Biomarkers in Embryo Development |
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Autors: | Gomez-Mingot, Maria | Griveau, Sophie | Bedioui, Fethi | Banks, Craig E. | Montiel, Vicente | Iniesta, Jesus |
Grups d'investigació o GITE: | Electroquímica Aplicada y Electrocatálisis |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Electroquímica |
Paraules clau: | Oxidative Stress | Human Embryo Development | Embryo Culture Medium | Radical Species | Electrochemical (Bio)sensors |
Àrees de coneixement: | Química Física |
Data de publicació: | 10-de setembre-2014 |
Editor: | Elsevier |
Citació bibliogràfica: | Electrochimica Acta. 2014, 140: 42-48. doi:10.1016/j.electacta.2014.03.012 |
Resum: | Oxidative stress has recently became one of the most important and negative factors that affect favourable human reproduction and therefore plays a crucial role in the successful outcome rates on artificial reproduction techniques (ARTs). The effects of oxidative stress are strengthened in an ART setting since there are more sources of reactive oxygen and nitrogen species (ROS/RNS) generation and a more vulnerable physiological defence mechanism. Culture media, for this reason, have improved in their composition and are becoming more complex, above everything, in their antioxidant power to face ROS/RNS generation. In this work, we suggest the use of electrochemical (bio)sensors to evaluate the presence and evolution of nitric oxide (NO) and superoxide anion (O2−), in a commercial culture medium compared to conventional phosphate buffer solution. The electrochemical monitoring of NO was followed by the real-time NO-release from a commercially available NO donor (diazeniumdiolate) under aerobic conditions in a surface-modified screen-printed linear platinum array electrode. Concurrently, superoxide anion detection was evaluated at a surface-modified screen-printed linear gold electrode by the real-time and in situ generation of O2−. The performances of both arrayed sensors were compared to those obtained at single modified ultramicroelectrodes. We deepen the study of the intrinsic and external interferents for the application of these electrochemical (bio)sensors in order to provide an oxidative stress index during embryo development. |
Patrocinadors: | M. Gómez-Mingot gratefully acklowledges the Universidad de Alicante for the PhD fellowship that allowed her to visit the laboratory in the group of Dr. Bedioui in Paris. Financial support from the Spanish Ministry of Science and Technology (“Project CTQ2010-18570”) and the University of Alicante are gratefully acknowledged. |
URI: | http://hdl.handle.net/10045/47220 |
ISSN: | 0013-4686 (Print) | 1873-3859 (Online) |
DOI: | 10.1016/j.electacta.2014.03.012 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © 2014 Published by Elsevier Ltd. |
Revisió científica: | si |
Versió de l'editor: | http://dx.doi.org/10.1016/j.electacta.2014.03.012 |
Apareix a la col·lecció: | INV - LEQA - Artículos de Revistas |
Arxius per aquest ítem:
Arxiu | Descripció | Tamany | Format | |
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2014_Gomez-Mingot_etal_ElectActa_final.pdf | Versión final (acceso restringido) | 821,68 kB | Adobe PDF | Obrir Sol·licitar una còpia |
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