PipX, the coactivator of NtcA, is a global regulator in cyanobacteria
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Título: | PipX, the coactivator of NtcA, is a global regulator in cyanobacteria |
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Autor/es: | Espinosa, Javier | Rodríguez, Francisco | Salinas, Paloma | Lanza, Val F. | Dixon, Ray | Cruz, Fernando de la | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias | Análisis de Datos y Modelización de Procesos en Biología y Geociencias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Departamento de Matemática Aplicada |
Palabras clave: | Nitrogen regulation | Transcription | Translation | Photosynthesis |
Área/s de conocimiento: | Genética | Matemática Aplicada |
Fecha de publicación: | 27-may-2014 |
Editor: | National Academy of Sciences |
Cita bibliográfica: | Proceedings of the National Academy of Sciences. 2014, 111(23): E2423-E2430. doi:10.1073/pnas.1404097111 |
Resumen: | To modulate the expression of genes involved in nitrogen assimilation, the cyanobacterial PII-interacting protein X (PipX) interacts with the global transcriptional regulator NtcA and the signal transduction protein PII, a protein found in all three domains of life as an integrator of signals of the nitrogen and carbon balance. PipX can form alternate complexes with NtcA and PII, and these interactions are stimulated and inhibited, respectively, by 2-oxoglutarate, providing a mechanistic link between PII signaling and NtcA-regulated gene expression. Here, we demonstrate that PipX is involved in a much wider interaction network. The effect of pipX alleles on transcript levels was studied by RNA sequencing of S. elongatus strains grown in the presence of either nitrate or ammonium, followed by multivariate analyses of relevant mutant/control comparisons. As a result of this process, 222 genes were classified into six coherent groups of differentially regulated genes, two of which, containing either NtcA-activated or NtcA-repressed genes, provided further insights into the function of NtcA–PipX complexes. The remaining four groups suggest the involvement of PipX in at least three NtcA-independent regulatory pathways. Our results pave the way to uncover new regulatory interactions and mechanisms in the control of gene expression in cyanobacteria. |
Patrocinador/es: | This work was supported by the Spanish Ministry of Economy and Competitivity (Grants BFU2009-07371, BFU2012-33364, and BFU2011-26608) and the European Seventh Framework Program (Grants 289326/FP7-KBBE-2011-5 and 282004/FP7-HEALTH-2011-2.3.1-2). |
URI: | http://hdl.handle.net/10045/44395 |
ISSN: | 1091-6490 |
DOI: | 10.1073/pnas.1404097111 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2014 National Academy of Sciences |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1073/pnas.1404097111 |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas INV - MODDE - Artículos de Revistas Investigaciones financiadas por la UE |
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