Chip-based capillary electrophoresis profiling of olive pollen extracts used for allergy diagnosis and immunotherapy

Abstract

Standardization of protein extracts for clinical purposes represents an important task in order to maintain adequate reactivity, presence of the relevant allergens and safety among other factors. The main objective of this work was to explore the potential use of a chip-based automated capillary electrophoresis system commercially available to analyze several of the most common forms of allergenic extracts from olive pollen used in allergy clinics. These include experimental extracts prepared from olive pollens, in-house reference extracts, extracts designed for skin prick test assays, and a panel of vaccine variants aimed to specific immunotherapy. As a major conclusion of the study, chip-based capillary electrophoresis allowed in all cases to determine accurate protein profiles with different degrees of sensitivity, where several allergens (particularly the major olive pollen allergen Ole e 1) were easily recognized. Moreover, several purified allergens were also analyzed by this method, and proposed as specific standards for different purposes. In the present condition, the method can only provide the protein profile of the extracts respect to a pre-established standard extract, but not allergen identification. However, these and other future developments and applications are discussed.