Culturable aerobic and facultative bacteria from the gut of the polyphagic dung beetle Thorectes lusitanicus Jeckel
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Título: | Culturable aerobic and facultative bacteria from the gut of the polyphagic dung beetle Thorectes lusitanicus Jeckel |
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Autor/es: | Hernández, Noemi | Escudero, José A. | San Millán, Álvaro | González Zorn, Bruno | Lobo, Jorge M. | Verdú, José R. | Suárez Rodríguez, Mónica |
Grupo/s de investigación o GITE: | Biodiversidad y Biotecnología aplicadas a la Biología de la Conservación |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias Ambientales y Recursos Naturales | Universidad de Alicante. Centro Iberoamericano de la Biodiversidad |
Palabras clave: | Actinobacteria | Proteobacteria | Firmicutes | Gut microbiota | Polyphagy | Geotrupidae |
Área/s de conocimiento: | Zoología |
Fecha de publicación: | 12-dic-2013 |
Editor: | John Wiley & Sons |
Cita bibliográfica: | Insect Science. 2015, 22(2): 178-190. doi:10.1111/1744-7917.12094 |
Resumen: | Unlike other dung beetles, the Iberian geotrupid Thorectes lusitanicus exhibits polyphagous behavior; for example, it is able to eat acorns, fungi, fruits, and carrion in addition to the dung of different mammals. This adaptation to digest a wider diet has physiological and developmental advantages and requires key changes in the composition and diversity of the beetle's gut microbiota. In this study, we isolated aerobic, facultative anaerobic, and aerotolerant microbiota amenable to grow in culture from the gut contents of T. lusitanicus and resolved isolate identity to the species level by sequencing 16S rRNA gene fragments. Using BLAST similarity searches and maximum likelihood phylogenetic analyses, we were able to reveal that the analyzed fraction (culturable, aerobic, facultative anaerobic, and aerotolerant) of beetle gut microbiota is dominated by the phyla Proteobacteria, Firmicutes and Actinobacteria. Among Proteobacteria, members of the order Enterobacteriales (Gammaproteobacteria) were the most abundant. The main functions associated with the bacteria found in the gut of T. lusitanicus would likely include nitrogen fixation, denitrification, detoxification, and diverse defensive roles against pathogens. |
Patrocinador/es: | This study was supported by the project 065/2002 of the Ministry of Environment, and the projects “Thorbellota” (CGL2008/03878/BOS) and “NiTerDist” (CGL2011-515 25544) of the Secretaría de Estado de Investigación, Desarrollo e Innovación. |
URI: | http://hdl.handle.net/10045/34638 |
ISSN: | 1672-9609 (Print) | 1744-7917 (Online) |
DOI: | 10.1111/1744-7917.12094 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | The definitive version is available at http://onlinelibrary.wiley.com/ |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1111/1744-7917.12094 |
Aparece en las colecciones: | INV - BBaBC - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2013_Hernandez_etal_InsectScience.pdf | Versión revisada (acceso abierto) | 1,55 MB | Adobe PDF | Abrir Vista previa |
2013_Hernandez_etal_InsectScience_final.pdf | Versión final (acceso restringido) | 404,78 kB | Adobe PDF | Abrir Solicitar una copia |
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