Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: implications for the detection of minimal residual disease in patients with small cell lung cancer
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Título: | Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: implications for the detection of minimal residual disease in patients with small cell lung cancer |
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Autor/es: | Torà, Montserrat | Barberá, Víctor Manuel | Real, Francisco X. |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | Reverse transcriptase-polymerase chain reaction | Small cell lung cancer | Neuroblastoma | HuD | Minimal residual disease |
Área/s de conocimiento: | Genética |
Fecha de publicación: | 20-dic-2000 |
Editor: | Elsevier |
Cita bibliográfica: | TORÀ, Montserrat; BARBERÁ, Víctor M.; REAL, F.X. "Detection of HuD transcripts by means of reverse transcriptase and polymerase chain reaction: implications for the detection of minimal residual disease in patients with small cell lung cancer". Cancer Letters. Vol. 161, No. 2 (20 Dec. 2000). ISSN 0304-3835, pp. 157-164 |
Resumen: | Small cell lung cancer (SCLC) expresses neuroectodermal markers including HuD, the best characterized member of the Hu gene family. The aim of this study is to optimize a simple and sensitive reverse transcriptase-polymerase chain reaction assay to detect circulating HuD-expressing cells for the early detection of SCLC recurrences. HuD-specific primers that selectively amplify the three HuD isoforms allowed the detection of one tumor cell/10(6) non-tumor cells. However, HuD transcripts were also detected in the mononuclear fraction of all samples from normal individuals (n=6) and patients with SCLC (n=5). By contrast, HuD protein was not detected in these fractions using Western blotting. More quantitative assays are necessary to examine the value of HuD transcript detection for the identification of tumor recurrences. |
Patrocinador/es: | Fondo de Investigación Sanitaria (92-0010) and Generalitat de Catalunya (SGR-00433). |
URI: | http://hdl.handle.net/10045/20251 |
ISSN: | 0304-3835 (Print) | 1872-7980 (Online) |
DOI: | 10.1016/S0304-3835(00)00577-2 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/S0304-3835(00)00577-2 |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas |
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