SipA, a novel type of protein from Synechococcus sp. PCC 7942, binds to the kinase domain of NblS
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/1728
Título: | SipA, a novel type of protein from Synechococcus sp. PCC 7942, binds to the kinase domain of NblS |
---|---|
Autor/es: | Espinosa, Javier | Fuentes, Inmaculada | Burillo Sanz, Sergio | Rodríguez, Francisco | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Universidad de Alicante. Departamento de Matemática Aplicada |
Palabras clave: | Two-hybrid | Protein interaction | Signal transduction | Bleaching | Regulation | NblS | SipA | Cyanobacteria | Histidine kinase | ATP-binding domain |
Área/s de conocimiento: | Genética |
Fecha de creación: | 2005 |
Fecha de publicación: | ene-2006 |
Editor: | Blackwell Publishing |
Cita bibliográfica: | ESPINOSA MANZANO, Javier, et al. “SipA, a novel type of protein from Synechococcus sp. PCC 7942, binds to the kinase domain of NblS”. FEMS Microbiology Letters. Vol. 254, Issue 1 (Jan. 2006). ISSN 0378-1097, pp. 41-47 |
Resumen: | Cyanobacteria respond to nutrient stress conditions by degrading their light-harvesting complexes for photosynthesis, a process regulated in Synechococcus sp. PCC 7942 by the sensor histidine kinase non-bleaching sensor (NblS). In yeast two-hybrid screenings for proteins interacting with NblS we have identified a novel type of protein, named SipA for NblS interacting protein A. Specific binding between NblS and SipA is observed with both yeast and bacterial two-hybrid systems. Additional yeast two-hybrid screenings with SipA as bait further confirmed the specificity of the interaction and allowed us to map their determinants to the ATP-binding domain of NblS. Strong conservation and coevolution of both NblS and SipA in cyanobacteria further suggests the importance of SipA in the context of the NblS signal transduction network. |
Patrocinador/es: | This work was supported by grants BMC2002-01156 from the Ministerio de Ciencia y Tecnología and GV04B-525 from the Generalitat Valenciana. |
URI: | http://hdl.handle.net/10045/1728 |
ISSN: | 0378-1097 |
DOI: | 10.1111/j.1574-6968.2005.00007.x |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | The definitive version is available at www.blackwell-synergy.com |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1111/j.1574-6968.2005.00007.x |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas INV - MODDE - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
SipArevised.pdf | Peer Review (acceso libre) | 451,71 kB | Adobe PDF | Abrir Vista previa |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.