Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization
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Campo DC | Valor | Idioma |
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dc.contributor | Biotecnología de Extremófilos (BIOTECEXTREM) | es_ES |
dc.contributor.author | Matarredona, Laura | - |
dc.contributor.author | García-Bonete, María José | - |
dc.contributor.author | Guío, Jorge | - |
dc.contributor.author | Camacho, Mónica | - |
dc.contributor.author | Fillat, María F. | - |
dc.contributor.author | Esclapez, Julia | - |
dc.contributor.author | Bonete, María-José | - |
dc.contributor.other | Universidad de Alicante. Departamento de Bioquímica y Biología Molecular y Edafología y Química Agrícola | es_ES |
dc.date.accessioned | 2024-01-26T12:36:49Z | - |
dc.date.available | 2024-01-26T12:36:49Z | - |
dc.date.issued | 2024-01-18 | - |
dc.identifier.citation | International Journal of Biological Macromolecules. 2024, 260(Part 2): 129541. https://doi.org/10.1016/j.ijbiomac.2024.129541 | es_ES |
dc.identifier.issn | 0141-8130 (Print) | - |
dc.identifier.issn | 1879-0003 (Online) | - |
dc.identifier.uri | http://hdl.handle.net/10045/140040 | - |
dc.description.abstract | Haloferax mediterranei, an extreme halophilic archaeon thriving in hypersaline environments, has acquired significant attention in biotechnological and biochemical research due to its remarkable ability to flourish in extreme salinity conditions. Transcription factors, essential in regulating diverse cellular processes, have become focal points in understanding its adaptability. This study delves into the role of the Lrp transcription factor, exploring its modulation of glnA, nasABC, and lrp gene promoters in vivo through β-galactosidase assays. Remarkably, our findings propose Lrp as the pioneering transcriptional regulator of nitrogen metabolism identified in a haloarchaeon. This study suggests its potential role in activating or repressing assimilatory pathway enzymes (GlnA and NasA). The interaction between Lrp and these promoters is analyzed using Electrophoretic Mobility Shift Assay and Differential Scanning Fluorimetry, highlighting l-glutamine's indispensable role in stabilizing the Lrp-DNA complex. Our research uncovers that halophilic Lrp forms octameric structures in the presence of l-glutamine. The study reveals the three-dimensional structure of the Lrp as a homodimer using X-ray crystallography, confirming this state in solution by Small-Angle X-ray Scattering. These findings illuminate the complex molecular mechanisms driving Hfx. mediterranei's nitrogen metabolism, offering valuable insights about its gene expression regulation and enriching our comprehension of extremophile biology. | es_ES |
dc.description.sponsorship | Funding for this research was provided by the Ministry of Economy and Competitiveness and FEDER (BIO2013-42921-P); “Programa Propio para el Fomento de la I + D + i del Vicerrectorado de Investigación y Transferencia de Conocimiento” of the University of Alicante (Spain) (GRE20-02-C; VIGROB-016). | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | © 2024 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | es_ES |
dc.subject | Lrp transcription factor | es_ES |
dc.subject | EMSA | es_ES |
dc.subject | Haloarchaea | es_ES |
dc.subject | X-ray crystallography | es_ES |
dc.subject | SAXS | es_ES |
dc.subject | DSF | es_ES |
dc.title | Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.peerreviewed | si | es_ES |
dc.identifier.doi | 10.1016/j.ijbiomac.2024.129541 | - |
dc.relation.publisherversion | https://doi.org/10.1016/j.ijbiomac.2024.129541 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2013-42921-P | es_ES |
Aparece en las colecciones: | INV - BIOTECEXTREM - Artículos de Revistas |
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Matarredona_etal_2024_IntJBiolMacromol.pdf | 8,97 MB | Adobe PDF | Abrir Vista previa | |
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