Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/139556
Título: | Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei |
---|---|
Autor/es: | Payá, Gloria | Bautista, Vanesa | Pastor-Soler, Sandra | Camacho, Mónica | Esclapez, Julia | Bonete, María-José |
Grupo/s de investigación o GITE: | Biotecnología de Extremófilos (BIOTECEXTREM) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Bioquímica y Biología Molecular y Edafología y Química Agrícola |
Palabras clave: | Like-Sm proteins | Hfq | Lsm | Nutritional stress conditions | Microarray |
Fecha de publicación: | 1-ene-2024 |
Editor: | MDPI |
Cita bibliográfica: | Payá G, Bautista V, Pastor-Soler S, Camacho M, Esclapez J, Bonete M-J. Analysis of Lsm Protein-Mediated Regulation in the Haloarchaeon Haloferax mediterranei. International Journal of Molecular Sciences. 2024; 25(1):580. https://doi.org/10.3390/ijms25010580 |
Resumen: | The Sm protein superfamily includes Sm, like-Sm (Lsm), and Hfq found in the Eukarya, Archaea, and Bacteria domains. Archaeal Lsm proteins have been shown to bind sRNAs and are probably involved in various cellular processes, suggesting a similar function in regulating sRNAs by Hfq in bacteria. Moreover, archaeal Lsm proteins probably represent the ancestral Lsm domain from which eukaryotic Sm proteins have evolved. In this work, Haloferax mediterranei was used as a model organism because it has been widely used to investigate the nitrogen cycle and its regulation in Haloarchaea. Predicting this protein’s secondary and tertiary structures has resulted in a three-dimensional model like the solved Lsm protein structure of Archaeoglobus fulgidus. To obtain information on the oligomerization state of the protein, homologous overexpression and purification by means of molecular exclusion chromatography have been performed. The results show that this protein can form hexameric complexes, which can aggregate into 6 or 12 hexameric rings depending on the NaCl concentration and without RNA. In addition, the study of transcriptional expression via microarrays has allowed us to obtain the target genes regulated by the Lsm protein under nutritional stress conditions: nitrogen or carbon starvation. Microarray analysis has shown the first universal stress proteins (USP) in this microorganism that mediate survival in situations of nitrogen deficiency. |
Patrocinador/es: | This research was funded by the Spanish Ministry of Economy and Competitiveness and FEDER (grant BIO2013-42921-P); the Generalitat Valenciana (Spain) (grant ACIF/2018/200); and the “Programa Propio para el Fomento de la I+D+i del Vicerrectorado de Investigación y Transferencia de Conocimiento” (VIGROB-016) of the University of Alicante (Spain). |
URI: | http://hdl.handle.net/10045/139556 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms25010580 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/ijms25010580 |
Aparece en las colecciones: | INV - BIOTECEXTREM - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Paya_etal_2024_IntJMolSci.pdf | 4,54 MB | Adobe PDF | Abrir Vista previa | |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.