IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa
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Título: | IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa |
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Autor/es: | Gómez-Torres, María José | Hernández-Falcó, Miranda | López-Botella, Andrea | Huerta-Retamal, Natalia | Sáez Espinosa, Paula |
Grupo/s de investigación o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología | Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología |
Palabras clave: | Acrosome reaction | Gamete fusion | IZUMO1 | Sperm selection | Male infertility | Immunofluorescent | Hyaluronic acid | Sperm capacitation |
Fecha de publicación: | 24-oct-2023 |
Editor: | MDPI |
Cita bibliográfica: | Gómez-Torres MJ, Hernández-Falcó M, López-Botella A, Huerta-Retamal N, Sáez-Espinosa P. IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa. Biomedicines. 2023; 11(11):2872. https://doi.org/10.3390/biomedicines11112872 |
Resumen: | IZUMO1 is an acrosome transmembrane protein implicated in the adhesion and fusion of gametes. This study aims to describe the distribution of IZUMO1 in human sperm under different physiological conditions: before capacitation (NCS), at one-hour capacitation (CS1), after a hyaluronic acid (HA) selection test (mature, MS1 and immature, IS1), and induced acrosome reaction from one-hour-capacitated sperm (ARS1). The data obtained in NCS, CS1, and MS1 significantly highlight dotted fluorescence in the acrosomal region (P1) as the major staining pattern (~70%). Moreover, we describe a new distribution pattern (P2) with a dotted acrosomal region and a labelled equatorial region that significantly increases in HA-bound spermatozoa, suggesting the onset of the migration of IZUMO1. In contrast, unbound spermatozoa presented an increase in P3 (equatorial region labelled) and P4 (not labelled). Finally, costaining to observe IZUMO1 distribution and acrosome status was performed in ARS1. Interestingly, we reported a variety of combinations between the IZUMO1 staining patterns and the acrosomal stages. In conclusion, these data show as a novelty the diffusion of the IZUMO1 protein during different physiological conditions that could contribute to the improvement in sperm selection techniques. |
Patrocinador/es: | This research was funded by Ministerio de Ciencia e Innovación (PID2021-123091NB-C22) and by Universidad de Alicante (VIGROB-186). |
URI: | http://hdl.handle.net/10045/138145 |
ISSN: | 2227-9059 |
DOI: | 10.3390/biomedicines11112872 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/biomedicines11112872 |
Aparece en las colecciones: | INV - Grupo de Inmunología - Artículos de Revistas |
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