Colostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics Technique, SWATH-MS
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Título: | Colostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics Technique, SWATH-MS |
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Autor/es: | Hernández-Caravaca, Iván | Moros-Nicolás, Carla | González-Brusi, Leopoldo | Romero de Ávila, María José | De Paco Matallana, Catalina | Pelegrín, Pablo | Castaño Molina, María Ángeles | Díaz-Meca, Lucía | Sánchez-Romero, Javier | Martínez Alarcón, Laura | Avilés, Manuel | Izquierdo-Rico, María José |
Grupo/s de investigación o GITE: | Grupo Balmis de Investigación en Historia de la Ciencia, Cuidados en Salud y Alimentación (BALMIS) | Salud Comunitaria (SALUD) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Enfermería Comunitaria, Medicina Preventiva y Salud Pública e Historia de la Ciencia |
Palabras clave: | Breastfeeding | Colostrum | SARS-CoV-2 | COVID-19 | Proteomics |
Fecha de publicación: | 21-ago-2023 |
Editor: | MDPI |
Cita bibliográfica: | Hernández-Caravaca I, Moros-Nicolás C, González-Brusi L, Romero de Ávila MJ, De Paco Matallana C, Pelegrín P, Castaño-Molina MÁ, Díaz-Meca L, Sánchez-Romero J, Martínez-Alarcón L, et al. Colostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics Technique, SWATH-MS. Children. 2023; 10(8):1423. https://doi.org/10.3390/children10081423 |
Resumen: | Colostrum performs nutritional, anti-inflammatory and anti-infective functions and promotes immune system formation and organ development. The new coronavirus, SARS-CoV-2, has generated concerns about viral transmission through human milk, with a lack of evidence about human milk’s protective effects against the infection. This study aimed at analyzing presence of the virus and at identifying the protein expression profile of human colostrum in active and COVID-19-recovered patients. Colostrum samples were collected from women with COVID-19 (n = 3), women recently recovered from the infection (n = 4), and non-infected women (n = 5). The samples were analyzed by means of RT-qPCR to determine presence of the virus and using SWATH-MS for proteomic analysis. Proteomic results were then analyzed using bioinformatic methods. The viral tests were negative for SARS-CoV-2 in the colostrum from COVID-19 patients. The proteomic analysis identified 301 common proteins in all samples analyzed. Nineteen proteins were upregulated and 7 were downregulated in the COVID-19 group versus the control samples, whereas 18 were upregulated and 7 were downregulated when comparing the COVID-19 group to the recovered group. Eleven proteins were biomarkers of active COVID-19 infection. Ten were upregulated: ACTN1, CD36, FAM3B, GPRC5B, IGHA2, IGK, PLTP, RAC1, SDCBP and SERPINF1, and one was downregulated: PSAP. These proteins are mainly related to immunity, inflammatory response and protein transport. In conclusion, the results of this study suggest that colostrum is not a vehicle for mother-to-child SARS-CoV-2 transmission. Moreover, the colostrum’s proteome of active and recuperated patients indicate that it could provide immune benefits to infants. |
Patrocinador/es: | This research was funded by Fundación Séneca (grants 20859/PI/18 and 0003/COVI/20). |
URI: | http://hdl.handle.net/10045/136762 |
ISSN: | 2227-9067 |
DOI: | 10.3390/children10081423 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/children10081423 |
Aparece en las colecciones: | INV - SALUD - Artículos de Revistas INV - BALMIS - Artículos de Revistas |
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