Each Cellular Compartment Has a Characteristic Protein Reactive Cysteine Ratio Determining Its Sensitivity to Oxidation
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Título: | Each Cellular Compartment Has a Characteristic Protein Reactive Cysteine Ratio Determining Its Sensitivity to Oxidation |
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Autor/es: | Neves, Ricardo Pires das | Chagoyen, Mónica | Martínez Lorente, Antonio | Íñiguez Lobeto, Carlos | Calatrava, Ana | Calabuig, Juana | Iborra, Francisco J. |
Grupo/s de investigación o GITE: | Biotecnología |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | Oxidative stress | Nuclear speckles | SMN | RNA polymerase | Transcription | 8-hydroxy guanosine | Thioredoxin | Oxidized RNA |
Fecha de publicación: | 14-jun-2023 |
Editor: | MDPI |
Cita bibliográfica: | Neves RPd, Chagoyen M, Martinez-Lorente A, Iñiguez C, Calatrava A, Calabuig J, Iborra FJ. Each Cellular Compartment Has a Characteristic Protein Reactive Cysteine Ratio Determining Its Sensitivity to Oxidation. Antioxidants. 2023; 12(6):1274. https://doi.org/10.3390/antiox12061274 |
Resumen: | Signaling and detoxification of Reactive Oxygen Species (ROS) are important patho-physiologcal processes. Despite this, we lack comprehensive information on individual cells and cellular structures and functions affected by ROS, which is essential to build quantitative models of the effects of ROS. The thiol groups from cysteines (Cys) in proteins play a major role in redox defense, signaling, and protein function. In this study, we show that the proteins in each subcellular compartment contain a characteristic Cys amount. Using a fluorescent assay for -SH in thiolate form and amino groups in proteins, we show that the thiolate content correlates with ROS sensitivity and signaling properties of each compartment. The highest absolute thiolate concentration was found in the nucleolus, followed by the nucleoplasm and cytoplasm whereas protein thiolate groups per protein showed an inverse pattern. In the nucleoplasm, protein reactive thiols concentrated in SC35 speckles, SMN, and the IBODY that accumulated oxidized RNA. Our findings have important functional consequences, and explain differential sensitivity to ROS. |
Patrocinador/es: | The Spanish Ministry of Science and Innovation supported this research under grant number: PID2019-111133RB-100. |
URI: | http://hdl.handle.net/10045/135476 |
ISSN: | 2076-3921 |
DOI: | 10.3390/antiox12061274 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/antiox12061274 |
Aparece en las colecciones: | INV - GIDBT - Artículos de Revistas |
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