Fluorescent Nanocomposite Hydrogels Based on Conjugated Polymer Nanoparticles as Platforms for Alkaline Phosphatase Detection
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Título: | Fluorescent Nanocomposite Hydrogels Based on Conjugated Polymer Nanoparticles as Platforms for Alkaline Phosphatase Detection |
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Autor/es: | Alacid, Yolanda | Esquembre, Rocío | Montilla, Francisco | Martínez-Tomé, María José | Mateo, C. Reyes |
Grupo/s de investigación o GITE: | Electrocatálisis y Electroquímica de Polímeros |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Nanocomposite hydrogel | Polyfluorene | Fluorescent sensor | Alkaline phosphatase | Immobilization | Portable device | Enzyme |
Fecha de publicación: | 21-mar-2023 |
Editor: | MDPI |
Cita bibliográfica: | Alacid Y, Esquembre R, Montilla F, Martínez-Tomé MJ, Mateo CR. Fluorescent Nanocomposite Hydrogels Based on Conjugated Polymer Nanoparticles as Platforms for Alkaline Phosphatase Detection. Biosensors. 2023; 13(3):408. https://doi.org/10.3390/bios13030408 |
Resumen: | This work describes the development and characterization of fluorescent nanocomposite hydrogels, with high swelling and absorption capacity, and prepared using a green protocol. These fluorescent materials are obtained by incorporating, for the first time, polyfluorenes-based nanoparticles with different emission bands—poly[9,9-dioctylfluorenyl-2,7-diyl] (PFO) and poly[(9,9-di-n-octylfluorenyl-2,7-diyl)-alt-(1,4-benzo-{2,1,3}-thiadiazole)] (F8BT)—into a three-dimensional polymeric network based on polyacrylamide. To this end, two strategies were explored: incorporation of the nanoparticles during the polymerization process (in situ) and embedment after the hydrogel formation (ex situ). The results show that the combination of PFO nanoparticles introduced by the ex situ method provided materials with good storage stability, homogeneity and reproducibility properties, allowing their preservation in the form of xerogel. The fluorescent nanocomposite hydrogels have been tested as a transportable and user-friendly sensing platform. In particular, the ability of these materials to specifically detect the enzyme alkaline phosphatase (ALP) has been evaluated as a proof-of-concept. The sensor was able to quantify the presence of the enzyme in an aqueous sample with a response time of 10 min and LOD of 21 nM. Given these results, we consider that this device shows great potential for quantifying physiological ALP levels as well as enzyme activity in environmental samples. |
Patrocinador/es: | This work was funded by the Spanish Ministry of Science and Innovation (TED2021-129894B-I00). The study forms part of the Advanced Materials programme and was supported by MCIN with funding from European Union NextGenerationEU (PRTR-C17.I1) and Generalitat Valenciana (MFA/2022/058). DLS, FESEM and Fluorescence lifetime spectrometer equipment acquisition funded by Generalitat Valenciana–Conselleria d’Educació Investigació Cultura i Esport and EUFEDER “Una forma de hacer Europa” (GVA-IDIFEDER/2018/020 and GVA-IDIFEDER/2021/036). |
URI: | http://hdl.handle.net/10045/133133 |
ISSN: | 2079-6374 |
DOI: | 10.3390/bios13030408 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/bios13030408 |
Aparece en las colecciones: | INV - GEPE - Artículos de Revistas |
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