Differences in gene expression patterns between cultured and natural Haloquadratum walsbyi ecotypes
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Título: | Differences in gene expression patterns between cultured and natural Haloquadratum walsbyi ecotypes |
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Autor/es: | Rosselli, Riccardo | López-Pérez, Mario | Martín Cuadrado, Ana Belén | Rodriguez-Valera, Francisco | Bolhuis, Henk |
Grupo/s de investigación o GITE: | Ecología Microbiana Molecular |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Palabras clave: | RNA-seq | Metatranscriptome | Solar saltern | Archaea | Haloquadratum walsbyi |
Fecha de publicación: | 10-nov-2022 |
Editor: | Frontiers Media |
Cita bibliográfica: | Rosselli R, López-Pérez M, Martin-Cuadrado A-B, Rodriguez-Valera F and Bolhuis H (2022) Differences in gene expression patterns between cultured and natural Haloquadratum walsbyi ecotypes. Front. Microbiol. 13:1044446. doi: 10.3389/fmicb.2022.1044446 |
Resumen: | Solar crystallizer ponds are characterized by high population density with a relatively simple community structure in terms of species composition. The microbial community in the solar saltern of Santa Pola (Alicante, Spain), is largely dominated by the hyperhalophilic square archaeon Haloquadratum walsbyi. Here we studied metatranscriptomes retrieved from a crystallizer pond during the winter of 2012 and summer of 2014 and compared Hqr. walsbyi’s transcription patterns with that of the cultured strain Hqr. walsbyi HBSQ001. Significant differences were found between natural and the cultured grown strain in the distribution of transcript levels per gene. This likely reflects the adaptation of the cultured strain to the relative homogeneous growth conditions while the natural species, which is represented by multiple ecotypes, is adapted to heterogeneous environmental conditions and challenges of nutrient competition, viral attack, and other stressors. An important consequence of this study is that expression patterns obtained under artificial cultivation conditions cannot be directly extrapolated to gene expression under natural conditions. Moreover, we found 195 significantly differential expressed genes between the seasons, with 140 genes being higher expressed in winter and mainly encode proteins involved in energy and carbon source acquiring processes, and in stress responses. |
Patrocinador/es: | RR received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 818431 (SIMBA). This output reflects only the author’s view, and the European Union cannot be held responsible for any use that may be made of the information contained therein. FRV was supported by grants “VIREVO” CGL2016-76273-P [MCI/AEI/FEDER, EU] (cofounded with FEDER funds) from the Spanish Ministerio de Ciencia e Innovación and “HIDRAS3” PROMETEU/2019/009 from Generalitat Valenciana. |
URI: | http://hdl.handle.net/10045/129388 |
ISSN: | 1664-302X |
DOI: | 10.3389/fmicb.2022.1044446 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2022 Rosselli, López-Pérez, Martin-Cuadrado, Rodriguez-Valera and Bolhuis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3389/fmicb.2022.1044446 |
Aparece en las colecciones: | INV - EMM - Artículos de Revistas Investigaciones financiadas por la UE |
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