Sperm Adhesion Molecule 1 (SPAM1) Distribution in Selected Human Sperm by Hyaluronic Acid Test
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Title: | Sperm Adhesion Molecule 1 (SPAM1) Distribution in Selected Human Sperm by Hyaluronic Acid Test |
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Authors: | Gómez-Torres, María José | Sáez Espinosa, Paula | Manzano-Santiago, Paula | Robles-Gómez, Laura | Huerta-Retamal, Natalia | Aizpurua, Jon |
Research Group/s: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Center, Department or Service: | Universidad de Alicante. Departamento de Biotecnología |
Keywords: | Hyaluronic acid | Hyaluronidase PH-20 | Male infertility | Spermatozoa | Sperm capacitation |
Issue Date: | 13-Oct-2022 |
Publisher: | MDPI |
Citation: | Gómez-Torres MJ, Sáez-Espinosa P, Manzano-Santiago P, Robles-Gómez L, Huerta-Retamal N, Aizpurua J. Sperm Adhesion Molecule 1 (SPAM1) Distribution in Selected Human Sperm by Hyaluronic Acid Test. Biomedicines. 2022; 10(10):2553. https://doi.org/10.3390/biomedicines10102553 |
Abstract: | The failures of binding to the oocyte zona pellucida are commonly attributed to defects in the sperm recognition, adhesion, and fusion molecules. SPAM1 (sperm adhesion molecule 1) is a hyaluronidase implicated in the dispersion of the cumulus-oocyte matrix. Therefore, the aim of this study was to characterize the SPAM1 distribution in the different physiological conditions of human sperm. Specifically, we evaluated the location of the SPAM1 protein in human sperm before capacitation, at one and four hours of capacitation and after hyaluronic acid (HA) selection test by fluorescence microscopy. Sperm bound to HA were considered mature and those that crossed it immature. Our results detected three SPAM1 fluorescent patterns: label throughout the head (P1), equatorial segment with acrosomal faith label (P2), and postacrosomal label (P3). The data obtained after recovering the mature sperm by the HA selection significantly (p < 0.05) highlighted the P1 in both capacitation times, being 79.74 and 81.48% after one hour and four hours, respectively. Thus, the HA test identified that human sperm require the presence of SPAM1 throughout the sperm head (P1) to properly contact the cumulus-oocyte matrix. Overall, our results provide novel insights into the physiological basis of sperm capacitation and could contribute to the improvement of selection techniques. |
Sponsor: | This research was funded by the Cátedra Human Fertility, Departamento de Biotecnología of the Universidad de Alicante (VIGROB-186) and «Proyectos de Generación de Conocimiento» within the framework of the State Program to Promote Scientific-Technical Research and its Transfer, of the State Plan for Scientific, Technical and Innovation Research 2017–2020 (PGC2018-094781-B-I00). |
URI: | http://hdl.handle.net/10045/129069 |
ISSN: | 2227-9059 |
DOI: | 10.3390/biomedicines10102553 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Peer Review: | si |
Publisher version: | https://doi.org/10.3390/biomedicines10102553 |
Appears in Collections: | INV - Grupo de Inmunología - Artículos de Revistas |
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