Screening of Relevant Metabolism-Disrupting Chemicals on Pancreatic β-Cells: Evaluation of Murine and Human In Vitro Models
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Title: | Screening of Relevant Metabolism-Disrupting Chemicals on Pancreatic β-Cells: Evaluation of Murine and Human In Vitro Models |
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Authors: | Al-Abdulla, Ruba | Ferrero, Hilda | Soriano, Sergi | Boronat-Belda, Talía | Alonso Magdalena, Paloma |
Research Group/s: | Fisiología Neuroendocrina (FINE) |
Center, Department or Service: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología |
Keywords: | Metabolism-disrupting chemicals | Pancreatic β-cell | Diabetes | Metabolic disorders | Insulin secretion | Electrical activity |
Knowledge Area: | Fisiología |
Issue Date: | 10-Apr-2022 |
Publisher: | MDPI |
Citation: | Al-Abdulla R, Ferrero H, Soriano S, Boronat-Belda T, Alonso-Magdalena P. Screening of Relevant Metabolism-Disrupting Chemicals on Pancreatic β-Cells: Evaluation of Murine and Human In Vitro Models. International Journal of Molecular Sciences. 2022; 23(8):4182. https://doi.org/10.3390/ijms23084182 |
Abstract: | Endocrine-disrupting chemicals (EDCs) are chemical substances that can interfere with the normal function of the endocrine system. EDCs are ubiquitous and can be found in a variety of consumer products such as food packaging materials, personal care and household products, plastic additives, and flame retardants. Over the last decade, the impact of EDCs on human health has been widely acknowledged as they have been associated with different endocrine diseases. Among them, a subset called metabolism-disrupting chemicals (MDCs) is able to promote metabolic changes that can lead to the development of metabolic disorders such as diabetes, obesity, hepatic steatosis, and metabolic syndrome, among others. Despite this, today, there are still no definitive and standardized in vitro tools to support the metabolic risk assessment of existing and emerging MDCs for regulatory purposes. Here, we evaluated the following two different pancreatic cell-based in vitro systems: the murine pancreatic β-cell line MIN6 as well as the human pancreatic β-cell line EndoC-βH1. Both were challenged with the following range of relevant concentrations of seven well-known EDCs: (bisphenol-A (BPA), bisphenol-S (BPS), bisphenol-F (BPF), perfluorooctanesulfonic acid (PFOS), di(2-ethylhexyl) phthalate (DEHP), cadmium chloride (CdCl2), and dichlorodiphenyldichloroethylene (DDE)). The screening revealed that most of the tested chemicals have detectable, deleterious effects on glucose-stimulated insulin release, insulin content, electrical activity, gene expression, and/or viability. Our data provide new molecular information on the direct effects of the selected chemicals on key aspects of pancreatic β-cell function, such as the stimulus-secretion coupling and ion channel activity. In addition, we found that, in general, the sensitivity and responses were comparable to those from other in vivo studies reported in the literature. Overall, our results suggest that both systems can serve as effective tools for the rapid screening of potential MDC effects on pancreatic β-cell physiology as well as for deciphering and better understanding the molecular mechanisms that underlie their action. |
Sponsor: | This study received funding from the European Union’s Horizon 2020 Research and Innovation programme under Grant agreement no 825712 (OBERON) project. The author’s laboratory also holds grant PID2020-113112RB-I00 funded by MCIN/AEI/10.13039/501100011033. CIBERDEM is an initiative of the Instituto de Salud Carlos III. |
URI: | http://hdl.handle.net/10045/123119 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms23084182 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Peer Review: | si |
Publisher version: | https://doi.org/10.3390/ijms23084182 |
Appears in Collections: | INV - FINE - Artículos de Revistas Research funded by the EU |
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Al-Abdulla_etal_2022_IntJMolSci.pdf | 46,26 MB | Adobe PDF | Open Preview | |
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