Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
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Title: | Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) |
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Authors: | Robles-Gómez, Laura | Sáez Espinosa, Paula | López-Viloria, Eliana Marina | López-Botella, Andrea | Aizpurua, Jon | Gómez-Torres, María José |
Research Group/s: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Center, Department or Service: | Universidad de Alicante. Departamento de Biotecnología |
Keywords: | Aleuria aurantia lectin | Capacitation | FE-SEM | Fucose | Glycoconjugates | Sperm |
Knowledge Area: | Biología Celular |
Issue Date: | 4-Nov-2021 |
Publisher: | MDPI |
Citation: | Robles-Gómez L, Sáez-Espinosa P, López-Viloria EM, López-Botella A, Aizpurua J, Gómez-Torres MJ. Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM). International Journal of Molecular Sciences. 2021; 22(21):11947. https://doi.org/10.3390/ijms222111947 |
Abstract: | The modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of terminal and linked fucose is still unknown. In this study, the quantitative and qualitative changes in fucosyl residues during different in vitro capacitation times (1 and 4 h), are simultaneously characterized by using Aleuria aurantia (AAA) lectin–gold labelling and high-resolution field emission scanning electron microscopy (FE-SEM) in human sperm. A significant decrease was found in the number of terminal fucose registered in the whole sperm head during the in vitro capacitation. Nevertheless, the quantification of fucose residues after 1 h of in vitro capacitation was very similar to those found after 4 h. Therefore, the changes observed in terminal and linked fucose during capacitation were not time-dependent. Furthermore, the comprehensive analysis of the topographic distribution showed the preferential fucosyl location in the acrosomal region and the presence of distinct clusters distributed over the head in all the studied conditions. Overall, these findings corroborate the validity of FE-SEM combined with gold labelling to register changes in surface molecules during in vitro sperm capacitation. |
Sponsor: | This research was funded by the Cátedra Human Fertility and Departamento de Biotecnología of the Universidad de Alicante (VIGROB-186). |
URI: | http://hdl.handle.net/10045/119170 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms222111947 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Peer Review: | si |
Publisher version: | https://doi.org/10.3390/ijms222111947 |
Appears in Collections: | INV - Grupo de Inmunología - Artículos de Revistas |
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