Characterization of Human Spermatic Subpopulations by ConA-Binding Sites and Tyrosine Phosphorylation during in vitro Capacitation and Acrosome Reaction
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Título: | Characterization of Human Spermatic Subpopulations by ConA-Binding Sites and Tyrosine Phosphorylation during in vitro Capacitation and Acrosome Reaction |
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Autor/es: | Sáez Espinosa, Paula | López-Huedo, Alba | Robles-Gómez, Laura | Huerta-Retamal, Natalia | Aizpurua, Jon | Gómez-Torres, María José |
Grupo/s de investigación o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | Acrosome reaction | Capacitation | ConA lectin | Spermatozoa | Tyrosine phosphorylation |
Área/s de conocimiento: | Biología Celular |
Fecha de publicación: | 19-abr-2021 |
Editor: | Karger |
Cita bibliográfica: | Cells Tissues Organs. 2021, 210: 1-9. https://doi.org/10.1159/000513275 |
Resumen: | Spermatozoa capacitation is a time-dependent physiological process essential for acquiring the fertilizing capacity. In this context, reorganization of spermatozoa surface sugars and tyrosine phosphorylation are some of the most important biochemical changes involved in capacitation. However, the relationship between these 2 biomarkers remains poorly defined. By cytofluorescence we simultaneously characterized the head concanavalin A (ConA)-binding sites and the flagellar tyrosine phosphorylation before capacitation, during different capacitation times (1 and 4 h), and after acrosome reaction induction in human spermatozoa. The results showed a strong connection between ConA-label patterns and tyrosine phosphorylation according to the spermatozoa capacitation time and acrosomal status. Specifically, the spermatozoa subpopulation with phosphotyrosine presented proper sugar location (label in acrosomal and postacrosomal region) just after 1 h of capacitation, while cells without phosphotyrosine needed 4 h to do it. Moreover, after induction of spermatozoa acrosome reaction, phosphorylation was significantly correlated (p < 0.05) with the relocation of ConA-binding residues to the equatorial region, regardless of capacitation time. Overall, these observations provide novel insights regarding spermatozoa subpopulations based on essential physiological events like capacitation and acrosome reaction, which could have potential implications in the improvement of spermatozoa selection techniques. |
Patrocinador/es: | This research was funded by the Human Fertility Cathedra of the University of Alicante and the VIGROB-186. |
URI: | http://hdl.handle.net/10045/116252 |
ISSN: | 1422-6405 (Print) | 1422-6421 (Online) |
DOI: | 10.1159/000513275 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 S. Karger AG, Basel |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1159/000513275 |
Aparece en las colecciones: | INV - Grupo de Inmunología - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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Saez-Espinosa_etal_2021_CellsTissuesOrgans_final.pdf | Versión final (acceso restringido) | 7,16 MB | Adobe PDF | Abrir Solicitar una copia |
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