Pyrroloquinoline quinone-dependent glucose dehydrogenase bioelectrodes based on one-step electrochemical entrapment over single-wall carbon nanotubes
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Título: | Pyrroloquinoline quinone-dependent glucose dehydrogenase bioelectrodes based on one-step electrochemical entrapment over single-wall carbon nanotubes |
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Autor/es: | Quintero-Jaime, Andrés Felipe | Conzuelo, Felipe | Cazorla-Amorós, Diego | Morallon, Emilia |
Grupo/s de investigación o GITE: | Electrocatálisis y Electroquímica de Polímeros | Materiales Carbonosos y Medio Ambiente |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Departamento de Química Inorgánica | Universidad de Alicante. Instituto Universitario de Materiales |
Palabras clave: | Entrapment | Carbon nanotubes | Phosphonic acid | PQQ-GDH | Biosensor | Glucose |
Área/s de conocimiento: | Química Física | Química Inorgánica |
Fecha de publicación: | 16-abr-2021 |
Editor: | Elsevier |
Cita bibliográfica: | Talanta. 2021, 232: 122386. https://doi.org/10.1016/j.talanta.2021.122386 |
Resumen: | Development of effective direct electron transfer is considered an interesting platform to obtain high performance bioelectrodes. Therefore, designing of scalable and cost-effective immobilization routes that promotes correct direct electrical contacting between the electrode material and the redox enzyme is still required. As we present here, electrochemical entrapment of pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on single-wall carbon nanotube (SWCNT)-modified electrodes was carried out in a single step during electrooxidation of para-aminophenyl phosphonic acid (4-APPA) to obtain active bioelectrodes. The adequate interaction between SWCNTs and the enzyme can be achieved by making use of phosphorus groups introduced during the electrochemical co-deposition of films, improving the electrocatalytic activity towards glucose oxidation. Two different procedures were investigated for electrode fabrication, namely the entrapment of reconstituted holoenzyme (PQQ-GDH) and the entrapment of apoenzyme (apo-GDH) followed by subsequent in situ reconstitution with the redox cofactor PQQ. In both cases, PQQ-GDH preserves its electrocatalytic activity towards glucose oxidation. Moreover, in comparison with a conventional drop-casting method, an important enhancement in sensitivity was obtained for glucose oxidation (981.7 ± 3.5 nA mM-1) using substantially lower amounts of enzyme and cofactor (PQQ). The single step electrochemical entrapment in presence of 4-APPA provides a simple method for the fabrication of enzymatic bioelectrodes. |
Patrocinador/es: | The authors would like to thank MICINN (PID2019-105923RB-I00) for the financial support. A.F.Q.J. gratefully acknowledges Generalitat Valenciana for the financial support through Santiago Grisolia grant (GRISOLIA/2016/084), to the University of Alicante for the support in the mobility program through the Escuela de Doctorado (EDUA). |
URI: | http://hdl.handle.net/10045/114416 |
ISSN: | 0039-9140 (Print) | 1873-3573 (Online) |
DOI: | 10.1016/j.talanta.2021.122386 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1016/j.talanta.2021.122386 |
Aparece en las colecciones: | INV - GEPE - Artículos de Revistas INV - MCMA - Artículos de Revistas |
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Quintero-Jaime_etal_2021_Talanta.pdf | 6,85 MB | Adobe PDF | Abrir Vista previa | |
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