Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM)
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Título: | Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM) |
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Autor/es: | Gómez-Torres, María José | Huerta-Retamal, Natalia | Robles-Gómez, Laura | Sáez Espinosa, Paula | Aizpurua, Jon | Avilés Sánchez, Manuel | Romero, Alejandro |
Grupo/s de investigación o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Biotecnología |
Palabras clave: | Spermatozoa | Capacitation | ARSA | FE-SEM | Gold-labeling |
Área/s de conocimiento: | Biología Celular |
Fecha de publicación: | 23-ene-2021 |
Editor: | MDPI |
Cita bibliográfica: | Gómez-Torres MJ, Huerta-Retamal N, Robles-Gómez L, Sáez-Espinosa P, Aizpurua J, Avilés M, Romero A. Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM). Cells. 2021; 10(2):222. https://doi.org/10.3390/cells10020222 |
Resumen: | Capacitation drives sperm biophysical and biochemical changes for sperm-oocyte interactions. It is a well-known fact that the molecular complex arylsulfatase A (ARSA), hyaluronidase sperm adhesion molecule 1 (SPAM1), and heat shock protein 2 (HSPA2) plays a significant role in sperm–zona pellucida (ZP) binding. However, the time-dependent capacitation effects on the sperm surface ARSA presence and specific topographic distributions remain to be elucidated. Here, we quantified the ARSA density and specific membrane domain locations before (US) and after in vitro capacitation (one and four hours; CS1–CS4) in human sperm using high-resolution field emission scanning electron microscopy (FE-SEM) and immunogold labeling. Our results showed a significant and progressive capacitation-mediated increase of labeled spermatozoa from the US (37%) to CS4 (100%) physiological conditions. In addition, surface mapping revealed a close relationship between the ARSA residues and their acrosomal repositioning. Compared with the ARSA surface heterogeneous distribution found in US, the CS1–4 conditions exhibited clustering on the peri-acrosomal region, showing that time-dependent capacitation also induced a ARSA residue dramatic translocation on sperm surfaces. Our findings provide novel insights into the molecular remodeling events preceding sperm-oocyte interactions. |
Patrocinador/es: | This research was funded by the Human Fertility Cathedra of the University of Alicante and R&D&I projects financed by competitive public entities (ViGrob-186, UAIND17-03 and PGC2018-094781-B-100). |
URI: | http://hdl.handle.net/10045/112703 |
ISSN: | 2073-4409 |
DOI: | 10.3390/cells10020222 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3390/cells10020222 |
Aparece en las colecciones: | INV - Grupo de Inmunología - Artículos de Revistas |
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