Human sperm chaperone HSPA2 distribution during in vitro capacitation
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http://hdl.handle.net/10045/110542
Títol: | Human sperm chaperone HSPA2 distribution during in vitro capacitation |
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Autors: | Huerta-Retamal, Natalia | Sáez Espinosa, Paula | Robles-Gómez, Laura | Avilés Sánchez, Manuel | Romero, Alejandro | Aizpurua, Jon | Gómez-Torres, María José |
Grups d'investigació o GITE: | Grupo de Inmunología, Biología Celular y del Desarrollo |
Centre, Departament o Servei: | Universidad de Alicante. Departamento de Biotecnología |
Paraules clau: | Capacitation | Heat shock protein A2 | HSP70 | Immunofluorescence | Spermatozoa |
Àrees de coneixement: | Biología Celular |
Data de publicació: | de febrer-2021 |
Editor: | Elsevier |
Citació bibliogràfica: | Journal of Reproductive Immunology. 2021, 143: 103246. https://doi.org/10.1016/j.jri.2020.103246 |
Resum: | Human fertilization success depends on the ability of the spermatozoa to undergo capacitation. Even though this process can be conducted in vitro, the optimal time for a sperm cell to complete capacitation in vitro is still under discussion due to the lack of proper capacitation biomarkers. Here, we evaluated the influence of in vitro capacitation time on HSPA2 distribution over human sperm head testing this chaperone as a potential capacitation biomarker. The chaperone was assessed in human spermatozoa from 16 normozoospermic donors using indirect immunofluorescence in uncapacitated, one and four-hour capacitated spermatozoa. The percentage of HSPA2 immunofluorescent cells before and after one hour of capacitation did not differ significantly. However, after four hours of capacitation, we observed a significantly higher percentage of HSPA2 labelled cells. In fluorescent cells analysed before capacitation, we could not identify a predominant distribution pattern. Meanwhile, after capacitation, most sperm showed a highly labelled equatorial band accompanied by a homogeneous fluorescence throughout the acrosomal region. Our findings suggest that HSPA2 needs more than one hour of in vitro capacitation for being correctly distributed in the anterior region of the sperm head. In conclusion, the present study provides solid evidences for the utility of HSPA2 as a biomarker of human sperm in vitro capacitation. Due to its importance during egg-sperm recognition, the use of HSPA2 as a biomarker before an artificial reproduction technique may be suggested, in addition to a longer capacitation time during sperm preparation. |
Patrocinadors: | This work was supported by the Human Fertility Cathedra of the University of Alicante and R&D&I projects financed by competitive public entities (ViGrob-186, UAIND17-03 and AGL2015). |
URI: | http://hdl.handle.net/10045/110542 |
ISSN: | 0165-0378 (Print) | 1872-7603 (Online) |
DOI: | 10.1016/j.jri.2020.103246 |
Idioma: | eng |
Tipus: | info:eu-repo/semantics/article |
Drets: | © 2020 Elsevier B.V. |
Revisió científica: | si |
Versió de l'editor: | https://doi.org/10.1016/j.jri.2020.103246 |
Apareix a la col·lecció: | INV - Grupo de Inmunología - Artículos de Revistas |
Arxius per aquest ítem:
Arxiu | Descripció | Tamany | Format | |
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Huerta-Retamal_etal_2021_JReprodImmun_final.pdf | Versión final (acceso restringido) | 1,03 MB | Adobe PDF | Obrir Sol·licitar una còpia |
Huerta-Retamal_etal_2021_JReprodImmun_accepted.pdf | Accepted Manuscript (acceso abierto) | 525,7 kB | Adobe PDF | Obrir Vista prèvia |
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