Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2
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Título: | Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2 |
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Autor/es: | Sahli, Kaouther | Gomri, Mohamed A. | Esclapez, Julia | Gómez‐Villegas, Patricia | Ghennai, Oulaya | Bonete, María-José | León, Rosa | Kharroub, Karima |
Grupo/s de investigación o GITE: | Biotecnología de Extremófilos (BIOTECEXTREM) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Palabras clave: | Algeria | Antibacterial | Antioxidant | Carotenoids | Haloarchaea |
Área/s de conocimiento: | Bioquímica y Biología Molecular |
Fecha de publicación: | jul-2020 |
Editor: | Wiley-VCH Verlag GmbH & Co. KGaA |
Cita bibliográfica: | Journal of Basic Microbiology. 2020, 60(7): 624-638. doi:10.1002/jobm.202000083 |
Resumen: | A set of 110 extremely halophilic archaeal strains were isolated from seven distinct saline habitats located in different regions of Algeria. The physicochemical characterization of the samples showed that these habitats were thalassohaline. The carotenoid production from isolated strains varied from 0.1 to 3.68 µg/ml. Based on their physiological characteristics and pigment production, 43 strains were selected and identified by means of phenotypic tests and 16S ribosomal RNA gene sequencing. Phylogenetic analysis indicated that the isolates corresponded to the class Halobacteria and were closely related to genera Halorubrum, Haloarcula, Haloferax, Natrinema, Halogeometricum, Haloterrigena , and Halopiger . Carotenoids of the highest producer, strain Halorubrum sp. BS2 were identified using high‐performance liquid chromatography–diode array detector and liquid chromatography–mass spectrometry. Bacterioruberin and bisanhydrobacterioruberin were the predominant carotenoids. The scavenging activity of these carotenoids reached 99% at a concentration of 18 μg/ml, which was much higher than that of ascorbic acid used as a reference compound. These carotenoids also exhibited significant antibacterial activities against four human‐pathogenic strains and four fish‐pathogenic strains. Variations in salinity, agitation rate, temperature, and light intensity were found to influence growth and carotenoid production of Halorubrum sp. BS2. Our results suggest that halophilic archaea represent a potential source for carotenoids, which are characterized by high antioxidant and antibacterial activities. |
Patrocinador/es: | This study was supported by the Algerian Ministry of Higher Education and Scientific Research, European Research Grant (INTERREG VA POCTEP‐055 ALGARED_PLUS_5E) and the group (VIGROB‐016) Universidad de Alicante. |
URI: | http://hdl.handle.net/10045/107940 |
ISSN: | 0233-111X (Print) | 1521-4028 (Online) |
DOI: | 10.1002/jobm.202000083 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2020 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1002/jobm.202000083 |
Aparece en las colecciones: | INV - BIOTECEXTREM - Artículos de Revistas |
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Sahli_etal_2020_JBasicMicrobiol_final.pdf | Versión final (acceso restringido) | 1,86 MB | Adobe PDF | Abrir Solicitar una copia |
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